Fibroblast Growth Factor-9 in Marsupial Testicular Development
Fibroblast Growth Factor-9 in Marsupial Testicular Development
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DOI：
10.1159/000327327
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发表时间：
2011-01-01
影响因子：
2.3
通讯作者：
Renfree, M. B.
中科院分区：
文献类型：
--
作者：
Chung, J. W.;Pask, A. J.;Renfree, M. B.
FGF9是成纤维细胞生长因子（FGF）家族的成员，对小鼠早期睾丸发育和生殖细胞存活至关重要。Fgf9增强睾丸决定因子Sox 9并拮抗卵巢因子Wnt 4。为了确定FGF9是否在哺乳动物性腺中具有保守的作用，我们研究了它在有袋动物，tammar wallaby Macropus eugenii性腺中的表达，并将其与WNT 4表达进行比较。有袋类FGF9与来自真兽目哺乳动物（包括人类）的直系同源物高度保守。FGF9蛋白在性分化前在睾丸和卵巢中均有表达，但在睾丸分化期出现性二态性。该蛋白质特异性地富集在支持细胞和生殖细胞中发育中睾丸的生精索中。FGF9 mRNA表达上调的tammar睾丸在生精索形成的时间和下调的发展中的卵巢在相反的配置文件有袋动物WNT4。这些观察结果表明，FGF9通过与WNT4的拮抗关系促进有袋动物早期性腺中的雄性命运，就像在真兽目哺乳动物中一样。版权所有（C）2011 S. Karger AG，巴塞尔
FGF9 is a member of the fibroblast growth factor (FGF) family and is critical for early testicular development and germ cell survival in the mouse. Fgf9 reinforces the testis determinant Sox9 and antagonizes Wnt4, an ovarian factor. To determine whether FGF9 has a conserved role in the mammalian gonad, we examined its expression in the gonads of a marsupial, the tammar wallaby Macropus eugenii, and compared it to WNT4 expression. Marsupial FGF9 is highly conserved with orthologues from eutherian mammals, including humans. FGF9 protein was detected in both the testis and ovary before sexual differentiation, but it subsequently became sexually dimorphic during the period of testicular differentiation. The protein was specifically enriched in the seminiferous cords of the developing testis in the Sertoli and germ cells. FGF9 mRNA expression was upregulated in the tammar testis at the time of seminiferous cord formation and down-regulated in the developing ovary in an opposite profile to that of marsupial WNT4. These observations suggest that FGF9 promotes male fate in the early gonad of marsupials through an antagonistic relationship with WNT4 as it does in eutherian mammals. Copyright (C) 2011 S. Karger AG, Basel