Pex19p, a Farnesylated Protein Essential for Peroxisome Biogenesis
Pex19p, a Farnesylated Protein Essential for Peroxisome Biogenesis
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Pex19p，一种对过氧化物酶体生物发生至关重要的法尼基化蛋白
DOI：
10.1128/mcb.18.1.616
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发表时间：
1998
影响因子：
5.3
通讯作者：
R. Erdmann
中科院分区：
文献类型：
--
作者：
K. Götte;W. Girzalsky;Michael Linkert;Evelyn Baumgart;Stefan Kammerer;W. Kunau;R. Erdmann
摘要我们报告了Pex 19 p的鉴定和分子特征，Pex 19 p是一种油酸诱导的法尼基化蛋白，分子量为39.7 kDa，是酿酒酵母过氧化物酶体生物合成所必需的。缺乏Pex 19 p的细胞的特征在于形态学上可检测的过氧化物酶体的缺乏和过氧化物酶体基质蛋白到胞质溶胶的错误定位。人HK 33基因产物被鉴定为Pex 19 p的推定的人直系同源物。提供的证据表明，法尼基化的Pex 19 p发生在C-末端CKQQ氨基酸序列的半胱氨酸。Pex 19 p的法尼基化被证明是过氧化物酶体生物合成中蛋白质的适当功能所必需的。Pex 19 p在体内与Pex 3 p相互作用，这种相互作用需要Pex 19 p的法尼基化。
ABSTRACT We report the identification and molecular characterization of Pex19p, an oleic acid-inducible, farnesylated protein of 39.7 kDa that is essential for peroxisome biogenesis in Saccharomyces cerevisiae. Cells lacking Pex19p are characterized by the absence of morphologically detectable peroxisomes and mislocalization of peroxisomal matrix proteins to the cytosol. The human HK33 gene product was identified as the putative human ortholog of Pex19p. Evidence is provided that farnesylation of Pex19p takes place at the cysteine of the C-terminal CKQQ amino acid sequence. Farnesylation of Pex19p was shown to be essential for the proper function of the protein in peroxisome biogenesis. Pex19p was shown to interact with Pex3p in vivo, and this interaction required farnesylation of Pex19p.
影响因子：
3.5
作者：
HORTON, RM;HUNT, HD;PEASE, LR
通讯作者：
PEASE, LR
DOI：
10.1083/jcb.114.6.1167
发表时间：
1991-09
期刊：
The Journal of cell biology
影响因子：
--
作者：
Höhfeld J;Veenhuis M;Kunau WH
通讯作者：
Kunau WH