Protein Kinase B Phosphorylation Correlates with Vascular Endothelial Growth Factor A and Microvessel Density in Gastric Adenocarcinoma
Protein Kinase B Phosphorylation Correlates with Vascular Endothelial Growth Factor A and Microvessel Density in Gastric Adenocarcinoma
复制标题
胃腺癌中蛋白激酶 B 磷酸化与血管内皮生长因子 A 和微血管密度相关
DOI：
10.1177/030006051204000610
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发表时间：
2012-11-01
影响因子：
1.6
通讯作者：
Lv, N. H.
中科院分区：
文献类型：
--
作者：
Zhou, X. D.;Chen, H. X.;Lv, N. H.
目的：信号分子蛋白激酶B(Akt)调节多种细胞过程。磷脂酰肌醇3-激酶(PI3K)/Akt信号通路在肿瘤血管生成中发挥重要作用。本研究旨在探讨磷酸化Akt在胃腺癌血管生成中的作用及其与血管内皮生长因子-A的相关性。方法：取手术切除的胃腺癌患者的肿瘤组织和配对的正常胃粘膜。Western blotting检测AKT和PAKT。免疫组织化学方法检测VEGF-A、PAKT和CD34的表达。结果：48例胃癌组织中AKT和PACK蛋白水平显著高于正常组织(n=48)。VEGF-A免疫染色阳性与PAKT免疫染色呈显著正相关。微血管密度与VEGF-A和PACT阳性表达呈正相关。结论：磷酸化Akt和VEGF-A参与了胃腺癌的血管生成，Akt的激活可能通过上调VEGF-A促进血管生成。PI3K/Akt/VEGF信号通路可能参与了胃腺癌的发生。
Objective: The signalling molecule protein kinase B (Akt) modulates many cellular processes. Phosphatidylinositol 3-kinase (PI3K)/Akt signalling pathways play important roles in tumour angiogenesis. The aim of this study was to determine the role of phosphorylated Akt (pAkt) in angiogenesis and its correlation with vascular endothelial growth factor (VEGF)-A in gastric adenocarcinoma. Methods: Tumour tissue and matched healthy gastric mucosa were obtained from patients undergoing surgical resection of gastric adenocarcinoma. Akt and pAkt were detected via Western blotting. VEGF-A, pAkt and CD34 were examined by immunohistochemistry. Results: Akt and pAkt protein levels were significantly higher in gastric cancer tissue than in normal tissue (n = 48 patients). Positive VEGF-A immuno -staining was significantly associated with pAkt immunostaining. Microvessel density was correlated with both VEGF-A and pAkt positivity. Conclusions: Phosphorylated Akt and VEGF-A are involved in angiogenesis of gastric adenocarcinoma, and Akt activation may contribute to angiogenesis via VEGF-A upregulation. The PI3K/Akt/VEGF signalling pathway may be involved in gastric adenocarcinoma.