Stabilization of neurites in cerebellar granule cells by transglutaminase activity: identification of midkine and galectin-3 as substrates
Stabilization of neurites in cerebellar granule cells by transglutaminase activity: identification of midkine and galectin-3 as substrates
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通过转谷氨酰胺酶活性稳定小脑颗粒细胞中的神经突：鉴定中期因子和半乳糖凝集素 3 作为底物
DOI：
10.1016/s0306-4522(00)00324-9
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发表时间：
2000
期刊：
影响因子：
3.3
通讯作者：
L. Haynes
中科院分区：
文献类型：
--
作者：
S. Mahoney;M. Wilkinson;S. Smith;L. Haynes
谷氨酰胺转胺酶C在细胞表面蛋白分子之间形成共价异肽交联，影响细胞的粘附和形态。类视黄酮诱导的交联活性在发育中的大鼠小脑皮层中存在[j]. et al.（1995）神经科学，65,1063-1076]。用单克隆抗体将转谷氨酰胺酶C定位于发育中的小脑皮质颗粒神经元。该酶在出生后培养的大鼠小脑皮层颗粒神经元和含有颗粒神经元前体的胚胎背菱形唇细胞中均可被维甲酸诱导。在生物基质基质上培养的活颗粒神经元中，研究了转谷氨酰胺酶活性的可能生物学功能，并使用转谷氨酰胺酶灭活剂RS-48373-007进行了延时电影分析。抑制交联活性不影响颗粒神经元形成的神经突的数量，但在最初的生长期间引起神经突的不稳定，被视为生长锥缩回数量的增加和过早轴突侧枝形成（分叉）的开始。只有当细胞在生物基质表面培养时，交联活性的失活才能阻止神经突间束的形成。参与细胞外基质相互作用的两种胶质蛋白，midkine和半乳糖凝集素-3，被确定为颗粒神经元转谷氨酰胺酶的推定底物。结果表明，由转谷氨酰胺酶C或相关酶形成的共价交联可产生胶质源性蛋白的多聚分子形式，并在稳定新形成的神经突中起作用。本文讨论了转谷氨酰胺酶在小脑皮层中通过发展颗粒神经元来控制轴突侧支的可能的非病理作用。
The formation of covalent isopeptide cross-links between cell surface protein molecules by the enzyme transglutaminase C influences cell adhesion and morphology. Retinoid-inducible cross-linking activity associated with this enzyme is present in the developing rat cerebellar cortex [Perry M. J. M. et al. (1995) Neuroscience65, 1063–1076]. A monoclonal antibody was used to localize transglutaminase C to granule neurons in the developing cerebellar cortex. The enzyme was inducible by retinoic acid both in granule neurons cultured from postnatal rat cerebellar cortex and in cells of the embryonic dorsal rhombic lip, which contain granule neuron precursors. A possible biological function for transglutaminase activity was investigated in living granule neurons, cultured on a biomatrix substratum, studied by time-lapse cinematographic analysis using the transglutaminase inactivator RS-48373-007. Inhibition of cross-linking activity did not influence the number of neurites formed by granule neurons, but caused the destabilization of neurites during the initial outgrowth period, seen as an increase in the number of growth cone retractions and the onset of premature axon collateral formation (bifurcation). Inactivation of cross-linking activity prevented the formation of fascicles between neurites only when cells were cultured on a biomatrix surface. Two glial proteins involved in cell–extracellular matrix interactions, midkine and galectin-3, were identified as putative substrates for granule neuron transglutaminase. The results suggest that covalent cross-link formation by transglutaminase C or a related enzyme generates multimeric molecular forms of glial-derived proteins, and plays a role in stabilizing newly formed neurites. A possible non-pathological role for transglutaminase in the control of axon collateral branching by developing granule neurons in the cerebellar cortex is discussed.
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碳水化合物结合蛋白 35 (Mac-2) 是一种层粘连蛋白结合凝集素，利用半胱氨酸 186 形成功能性二聚体。
DOI：
--
发表时间：
1991
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Woo,HJ;Lotz,MM;Jung,JU;Mercurio,AM
通讯作者：
Mercurio,AM
影响因子：
11.2
作者：
Hidenori Inohara;Shiro Akahani;K. Koths;A. Raz
通讯作者：
Hidenori Inohara;Shiro Akahani;K. Koths;A. Raz
影响因子：
2.9
作者：
Liu, FT;Hsu, DK;Chen, SS
通讯作者：
Chen, SS
影响因子：
4.6
作者：
G. Fishell;M. Hatten
通讯作者：
G. Fishell;M. Hatten
影响因子：
4.1
作者：
Rosenblatt, S;Bassuk, JA;Preissner, KT
通讯作者：
Preissner, KT