Gryllotalpicola reticulitermitis sp nov., isolated from a termite gut
Gryllotalpicola reticulitermitis sp nov., isolated from a termite gut
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Gryllotalpicola reticulitermitis sp nov.，从白蚁肠道中分离出来
DOI：
10.1099/ijs.0.062984-0
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发表时间：
2015-01-01
影响因子：
2.8
通讯作者：
Yang, Hong
中科院分区：
文献类型：
--
作者：
Fang, Hao;Lv, Wanyu;Yang, Hong
菌株TS-56(T)从中国白蚁（Reticulitermes chinensis Snyder）肠道中分离得到。基于16S rRNA基因序列的系统发育分析表明，该菌株属于微细菌科Gryllotalpicola属，与该属其他种的序列相似性为96.6% ~ 97.8%。革兰氏染色阳性，无运动，菌落呈浅黄色，呈不规则短杆状细胞（直径0.4 ~ 0.6 μ m，长度0.6 ~ 1.0 μ m）。TS-56(T)在20 ~ 35℃（最适30℃）和pH 4.0 ~ 8.0（最适pH 5.0）条件下生长。TS-56(T)的肽聚糖含有鸟氨酸、谷氨酸、丙氨酸、同型丝氨酸和甘氨酸。酰基型为乙酰基。TS-56(T)细胞脂肪酸含量最高的是环己基c -17:0（88.79%）。呼吸道甲萘醌为MK-11。极性脂质谱包含二磷脂酰甘油、磷脂酰甘油、磷脂酰胆碱、磷脂酰肌醇和两种未知的糖脂。该型菌株DNA中G+C含量为67.4 molok。基于系统发育特征和表型特征，TS-56(T)为Gryllotalpicola属新种，命名为Gryllotalpicola reticulitermitis sp. nov。型应变为TS-56(T) (=CGMCC 1.10363（T)=NBRC 109838(T)）。
Strain TS-56(T) was isolated from the gut of a wood-feeding termite, Reticulitermes chinensis Snyder. Phylogenetic analyses based on 16S rRNA gene sequences revealed that the strain represented a member of the genus Gryllotalpicola of the family Microbacteriaceae, with sequence similarities to other species of the genus ranging from 96.6% to 97.8%. The isolate was Gram-stain-positive, non-motile, with light yellow colonies and irregular short rod-shaped cells (0.4-0.6 mu m in diameter, 0.6-1.0 mu m in length). Growth of TS-56(T) occurred at 20-35 degrees C (optimum, 30 degrees C) and at pH 4.0-8.0 (optimum, pH 5.0). The peptidoglycan of TS-56(T) contained ornithine, glutamic acid, alanine, homoserine and glycine. The acyl type was acetyl. The most abundant cellular fatty acid of TS-56(T) was cyclohexyl-C-17:0 (88.79%). The respiratory menaquinone was MK-11. The polar lipid profile contained disphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, phosphatidylinositol and two unknown glycolipids. DNA of the type strain had a G+C content of 67.4 molok. On the basis of the phylogenetic properties and phenotypic distinctiveness, TS-56(T) represents a novel species of the genus Gryllotalpicola, for which the name Gryllotalpicola reticulitermitis sp. nov. is proposed. The type strain is TS-56(T) (=CGMCC 1.10363(T)=NBRC 109838(T)).