Gut microbiota differences in elderly subjects between rural city Kyotango and urban city Kyoto: an age-gender-matched study
Gut microbiota differences in elderly subjects between rural city Kyotango and urban city Kyoto: an age-gender-matched study
复制标题
DOI：
10.3164/jcbn.19-26
复制
发表时间：
2019-09-01
影响因子：
2.4
通讯作者：
Matoba, Satoaki
中科院分区：
文献类型：
--
作者：
Naito, Yuji;Takagi, Tomohisa;Matoba, Satoaki
关于肠道微生物群在促进健康和预防疾病方面的作用，已经报告了几项成果。京丹后市位于日本京都府的北方，是长寿地区之一，有各种各样的百岁老人。为了了解肠道菌群与城市化之间的关系，我们比较了京丹后市和京都城市之间老年健康受试者肠道菌群的多样性、丰度和功能;京都是位于京都府南部的一个城市。在京丹后市和京都共招募了51名年龄和性别匹配的受试者，并获得了他们的粪便样本，以使用165 rRNA基因测序分析肠道微生物群。β多样性的主坐标分析显示，两个城市之间的肠道微生物群存在显着差异。相比之下，α多样性分析显示各组之间没有显著差异。在门的水平上比较，厚壁菌门的丰度随着城市化而降低，而变形菌门和拟杆菌门的丰度则随着城市化而增加。在属水平上的比较，随着城市化，在毛螺菌科家庭，包括属Roseburia和粪球菌，观察到显着减少，并观察到在拟杆菌，颤螺旋，副拟杆菌，瘤胃球菌显着增加。随着城市化进程的加快，脂多糖合成蛋白和脂多糖合成途径的功能增强，转运蛋白和ABC转运蛋白的功能减弱。总之，目前的研究结果表明，在京都县的省会城市和城市之间的肠道微生物群的显着差异。这些微生物群的改变可能为考虑长寿与肠道微生物群之间的关系提供新的见解。
Several outcomes have been reported on the role of gut microbiota in health promotion and disease prevention. Kyotango, one of the longevity areas with various centenarians, is a provincial city located in the northern part of Kyoto Prefecture in Japan. To understand the relationship between gut microbiota and urbanization, we compared the diversity, abundance, and function of gut microbiota in older healthy subjects between Kyotango and Kyoto cities; Kyoto is an urban city located in the southern part of Kyoto Prefecture. In total, 51 subjects at Kyotango and 51 subjects at Kyoto matched by age and gender were recruited, and their fecal samples were obtained to analyze the gut microbiota using 165 rRNA gene sequencing. Principal coordinate analysis for beta-diversity revealed significant differences in the gut microbiota between two cities. In contrast, the analysis of alpha-diversity revealed no significant differences between the groups. On comparison at the phylum levels, the abundance of Firmicutes was decreased with the urbanization, whereas that of Proteobacteria and Bacteroidetes increased. On comparison at the genus levels, with urbanization, a significant decrease was observed in Lachnospiraceae families including genus Roseburia and Coprococcus, and significant increases was observed in Bacteroides, Oscillospira, Parabacteroides, and Ruminococcus. The most markedly increased functional pathway with urbanization was lipopolysaccharide biosynthesis proteins and lipopolysaccharide biosynthesis, and decreased pathway was transporters and ABC transporters. In conclusion, the present findings indicate significant differences in the gut microbiota between the provincial city and urban cities at Kyoto Prefecture. These alterations in the microbiota may provide new insights to consider the relationship between longevity and gut microbiota.