The monoaminergic modulation of sensory-mediated aversive responses in Caenorhabditis elegans requires glutamatergic/peptidergic cotransmission.
The monoaminergic modulation of sensory-mediated aversive responses in Caenorhabditis elegans requires glutamatergic/peptidergic cotransmission.
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DOI：
10.1523/jneurosci.0497-10.2010
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发表时间：
2010-06-09
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影响因子：
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通讯作者：
Komuniecki RW
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文献类型：
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作者：
Harris G;Mills H;Wragg R;Hapiak V;Castelletto M;Korchnak A;Komuniecki RW
单胺和神经肽相互作用调节脊椎动物和无脊椎动物的行为可塑性。在秀丽隐杆线虫中，行为状态或“情绪”依赖于食物的可用性，并且在大多数行为的微调中通过单胺能和肽能信号传导来翻译。在本研究中，我们研究了单胺和肽对C。由一对多模态、伤害性感受的ASH感觉神经元介导的线虫厌恶行为。食物或5-羟色胺通过一个需要nlp-3编码的神经肽从ASHs释放的途径使ASHs敏感并刺激厌恶反应。由nlp-3编码的肽似乎通过NPR-17受体刺激ASH介导的厌恶行为。NLP-3和NPR-17缺失动物表现出相同的表型，并且过表达NLP-3或NPR-17的动物表现出升高的对食物的厌恶反应，当NLP-3或NPR-17分别在NPR-17或NLP-3缺失动物中过表达时，这些反应是不存在的。通过激活ASHs中的Gαq或Gαs增加ASHs介导的厌恶反应，其中Gαs信号传导特异性刺激nlp-3编码肽的释放。相反，章鱼胺似乎通过激活ASHs中的Gαo信号传导来抑制5-HT刺激，这反过来又抑制Gαs和Gαq信号传导以及nlp-3编码肽的释放。这些结果表明，5-羟色胺和章鱼胺可逆地调节ASHs的活性，并突出了C。elegans模型，用于定义复杂感觉介导回路的单个神经元中单胺和肽之间的相互作用。
Monoamines and neuropeptides interact to modulate behavioral plasticity in both vertebrates and invertebrates. In Caenorhabditis elegans behavioral state or “mood” is dependent on food availability and is translated by both monoaminergic and peptidergic signaling in the fine-tuning of most behaviors. In the present study, we have examined the interaction of monoamines and peptides on C. elegans aversive behavior mediated by a pair of polymodal, nociceptive, ASH sensory neurons. Food or serotonin sensitize the ASHs and stimulate aversive responses through a pathway requiring the release of nlp-3 encoded neuropeptides from the ASHs. Peptides encoded by nlp-3 appear to stimulate ASH-mediated aversive behavior through the NPR-17 receptor. nlp-3 and npr-17 null animals exhibit identical phenotypes and animals over-expressing either nlp-3 or npr-17 exhibit elevated aversive responses off food that are absent when nlp-3 or npr-17 are overexpressed in npr-17 or nlp-3 null animals, respectively. ASH-mediated aversive responses are increased by activating either Gαq or Gαs in the ASHs, with Gαs signaling specifically stimulating the release of nlp-3 encoded peptides. In contrast, octopamine appears to inhibit 5-HT stimulation by activating Gαo signaling in the ASHs that, in turn, inhibits both Gαs and Gαq signaling and the release of nlp-3 encoded peptides. These results demonstrate that serotonin and octopamine reversibly modulate the activity of the ASHs, and highlight the utility of the C. elegans model for defining interactions between monoamines and peptides in individual neurons of complex sensory-mediated circuits.