Naked DNA delivered intraportally expresses efficiently in hepatocytes.
Naked DNA delivered intraportally expresses efficiently in hepatocytes.
复制标题
门脉内递送的裸DNA在肝细胞中有效表达。
作者：
V. Budker;Guofeng Zhang;S. Knechtle;J. Wolff
将高渗溶液中的裸质粒DNA注入肝静脉短暂阻断的小鼠的门静脉内。使用100微克的相应质粒载体，在全肝1%的肝细胞中实现了高水平的荧光素酶表达和β-半乳糖苷酶表达。门脉内注射100微克pCMVGH两天后，平均hGH血清浓度为65 ng/ml+/-26(n=7)，约为正常基线水平的50倍。这些来自裸质粒DNA的前所未有的外源基因表达水平证明了体内实质细胞在血管内传递后吸收裸露DNA的能力。
Naked plasmid DNA in hypertonic solutions was injected intraportally in mice whose hepatic veins were transiently occluded. High levels of luciferase expression and beta-galactosidase expression in 1% of the hepatocytes throughout the entire liver were achieved using 100 micrograms of the respective plasmid vector. Two days after the intraportal injection of 100 micrograms of pCMVGH, the mean hGH serum concentration was 65 ng/ml +/- 26 (n = 7) which is approximately 50-fold above normal baseline levels. These unprecedented levels of foreign gene expression from naked plasmid DNA document the ability of parenchymal cells in vivo to take up naked DNA following intravascular delivery.