Mycoviruses infecting the endophytic and entomopathogenic fungus Tolypocladium cylindrosporum
Mycoviruses infecting the endophytic and entomopathogenic fungus Tolypocladium cylindrosporum
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DOI：
10.1016/j.virusres.2011.06.015
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发表时间：
2011-09-01
期刊：
影响因子：
5
通讯作者：
Zabalgogeazcoa, Inigo
中科院分区：
文献类型：
--
作者：
Herrero, Noemi;Zabalgogeazcoa, Inigo
通过研究几种双链RNA（dsRNA）元件向分生孢子的传递，推断出内生和昆虫病原真菌Tolypocladium cylindrosporum菌株中的混合病毒感染。每种dsRNA的传播速率不同，并且获得了具有六种dsRNA的原始集合的不同组合的单孢子分离物。对一个5196 bp的dsRNA元件进行了测序，该元件代表了T.圆柱孢子病毒1（TcV 1）是全病毒科中的一个新成员。该病毒传播到81.4%的分生孢子;相比之下，4个3.1-3.7 kbp的dsRNA仅传播到从感染的亲本菌株获得的单孢子分离物的4.7%。这四个dsRNA在传播过程中没有分离，其中一个通过序列分析显示编码RdRp，这表明这四个分子可能代表了四分体黄病毒的整个基因组。第三种可能的病毒，基因组约为4.2 kbp，被传输到79.1%的单孢菌株所产生的感染菌株。利巴韦林治疗T. Cylindrosporum对TcV 1敏感。用80和100 μ M浓度的药物处理的感染菌株的所有单孢子培养物均不含TcV 1。(C)2011 Elsevier B. V.保留所有权利。
A mixed virus infection in a strain of the endophytic and entomopathogenic fungus Tolypocladium cylindrosporum was deduced from a study of the transmission to conidia of several double-stranded RNA (dsRNA) elements. The transmission rates of each dsRNA were different, and monosporic isolates harbouring different combinations of the original set of six dsRNAs were obtained. A 5196 bp dsRNA element was sequenced and represents the genome of T. cylindrosporum virus 1 (TcV1), a new member of the genus Victorivirus in the Totiviridae family. This virus was transmitted to 81.4% of the conidia; in contrast, four dsRNAs of 3.1-3.7 kbp were transmitted only to 4.7% of the monosporic isolates obtained from the infected parental strain. These four dsRNAs did not show segregation during transmission, and one of them was shown by sequence analysis to encode an RdRp, suggesting that the four molecules might represent the whole genome of a quadripartite chrysovirus. A third possible virus with a genome of approximately 4.2 kbp was transmitted to 79.1% of the monosporic isolates produced by the infected strain. Ribavirin was used to cure T. cylindrosporum from viruses, and TcV1 was sensitive to this drug. All monosporic cultures derived from the infected strain treated with 80 and 100 mu M concentrations of the drug were free of TcV1. (C) 2011 Elsevier B.V. All rights reserved.