Damaging effects of chronic low-dose methotrexate usage on primary bone formation in young rats and potential protective effects of folinic acid supplementary treatment
Damaging effects of chronic low-dose methotrexate usage on primary bone formation in young rats and potential protective effects of folinic acid supplementary treatment
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DOI：
10.1016/j.bone.2008.09.014
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发表时间：
2009-01-01
期刊：
影响因子：
4.1
通讯作者：
Xian, Cory J.
中科院分区：
文献类型：
--
作者：
Fan, Chiaming;Cool, Johanna C.;Xian, Cory J.
甲氨蝶呤 (MTX) 是癌症治疗和抗风湿药物中最常用的抗代谢药物。虽然已知高剂量的 MTX 化疗会导致骨骼生长缺陷，但长期使用低剂量的 MTX 化疗对骨骼生长的影响尚不清楚。在这里，我们检查了低剂量长期 MTX 化疗对患病幼鼠骨生长的影响，以及解毒剂亚叶酸（MTX 6 小时后 6 小时腹腔注射 1 mg/kg）补充治疗的潜在保护作用。经过两个周期的 5 次每日一次 MTX 注射（0.75 mg/kg，注射 5 天/注射 9 天/注射 5 天）后，组织学分析表明，与对照组相比，该剂量的 MTX 在第 22 天导致生长板和初级海绵骨高度显着降低（P
Methotrexate (MTX) is a most commonly used anti-metabolite in cancer treatment and as an anti-rheumatic drug. While MTX chemotherapy at a high dose is known to cause bone growth defects in growing bones, effects of its chronic use at a low dose on growing skeleton remain less clear. Here, we examined effects on bone growth of long-term MTX chemotherapy at a low dose ill Young rats, and potential protective effects of supplementary treatment with antidote folinic acid (given ip at 1 mg/kg 6 h after MTX). After two cycles of 5 once-daily MTX injections (at 0,75 mg/kg, 5 days on/9 days off/5 days on), histological analysis showed that MTX at this dose caused significant reduction in heights of growth plate and primary spongiosa bone on day 22 compared to controls (P