Designer deletion strains derived from Saccharomyces cerevisiae S288C: A useful set of strains and plasmids for PCR‐mediated gene disruption and other applications
Designer deletion strains derived from Saccharomyces cerevisiae S288C: A useful set of strains and plasmids for PCR‐mediated gene disruption and other applications
复制标题
DOI：
10.1002/(sici)1097-0061(19980130)14:2
复制
发表时间：
1998-01
期刊：
影响因子：
2.6
通讯作者：
Carrie Baker Brachmann;A. Davies;G. Cost;E. Caputo;Joachim Li;P. Hieter;J. Boeke
中科院分区：
文献类型：
--
作者：
Carrie Baker Brachmann;A. Davies;G. Cost;E. Caputo;Joachim Li;P. Hieter;J. Boeke
以酿酒酵母S288C为基础，根据酵母基因组序列设计删除常用的选择标记基因，构建了一套酵母菌株，并对其进行了分析。这些菌株最小化或消除了与常用载体中相应标记基因的同源性，而不会显著影响相邻基因的表达。由于常用的营养缺陷型标记基因片段和基因组序列之间的同源性已被大部分或完全消除，这些菌株也将减少质粒整合事件，质粒整合事件可干扰多种分子遗传学应用。我们还报道了pRS 400系列载体的新成员的构建，其含有kanMX、ADE 2和MET 15基因。© 1998 John Wiley & Sons，Ltd。
A set of yeast strains based on Saccharomyces cerevisiae S288C in which commonly used selectable marker genes are deleted by design based on the yeast genome sequence has been constructed and analysed. These strains minimize or eliminate the homology to the corresponding marker genes in commonly used vectors without significantly affecting adjacent gene expression. Because the homology between commonly used auxotrophic marker gene segments and genomic sequences has been largely or completely abolished, these strains will also reduce plasmid integration events which can interfere with a wide variety of molecular genetic applications. We also report the construction of new members of the pRS400 series of vectors, containing the kanMX, ADE2 and MET15 genes. © 1998 John Wiley & Sons, Ltd.