Differences in anatomical connections across distinct areas in the rodent prefrontal cortex
Differences in anatomical connections across distinct areas in the rodent prefrontal cortex
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DOI：
10.1111/ejn.13521
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发表时间：
2017-03-01
影响因子：
3.4
通讯作者：
Tinsley, Chris J.
中科院分区：
文献类型：
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作者：
Bedwell, Stacey A.;Billett, E. Ellen;Tinsley, Chris J.
前额叶皮质(PFC)网络结构与许多复杂的高阶功能有关，并与一系列神经功能障碍有关。因此，了解PFC的解剖连接对我们理解PFC的功能是至关重要的。在这里，我们将荧光金和氟红宝石注射到整个大鼠PFC的相同部位。在PFC内的两个冠状面(前缘+4.7 mm和后缘+3.7 mm)进行示踪剂注射。在每个冠状平面内，示踪剂被放置在相隔约1毫米的位置，并平行于皮质的内侧和眼眶表面。我们发现，荧光金和氟红宝石注射都在颞叶和感觉运动皮质产生了显著的标记。荧光金产生逆行标记，氟红宝石产生大量顺行标记。对这些连接在颞叶和感觉-运动皮质内的位置的分析显示了一个一致的拓扑结构(因为注射顺序是沿着每个冠状水平的眼眶表面向内侧进行的)。在前冠水平，注射产生了与我们实验室早期研究中在中央PFC看到的相似的拓扑结构(即比较使用相同示踪剂的同等位置的注射)，这在颞叶皮质中尤其明显。然而，在后冠水平，这种连接模式明显不同，显示出更高水平的互惠，在颞叶皮质和感觉-运动皮质。我们的发现表明，与前部相比，来自后部的联系的相对组织发生了变化，这可能为确定复杂过程的组织方式提供了基础。
Prefrontal cortex (PFC) network structure is implicated in a number of complex higher-order functions and with a range of neurological disorders. It is therefore vital to our understanding of PFC function to gain an understanding of its underlying anatomical connectivity. Here, we injected Fluoro-Gold and Fluoro-Ruby into the same sites throughout rat PFC. Tracer injections were applied to two coronal levels within the PFC (anterior +4.7 mm to bregma and posterior +3.7 mm to bregma). Within each coronal level, tracers were deposited at sites separated by approximately 1 mm and located parallel to the medial and orbital surface of the cortex. We found that both Fluoro-Gold and Fluoro-Ruby injections produced prominent labelling in temporal and sensory-motor cortex. Fluoro-Gold produced retrograde labelling and Fluoro-Ruby largely produced anterograde labelling. Analysis of the location of these connections within temporal and sensory-motor cortex revealed a consistent topology (as the sequence of injections was followed mediolaterally along the orbital surface of each coronal level). At the anterior coronal level, injections produced a similar topology to that seen in central PFC in earlier studies from our laboratory (i.e. comparing equivalently located injections employing the same tracer), this was particularly prominent within temporal cortex. However, at the posterior coronal level this pattern of connections differed significantly, revealing higher levels of reciprocity, in both temporal cortex and sensory-motor cortex. Our findings indicate changes in the relative organization of connections arising from posterior in comparison to anterior regions of PFC, which may provide a basis to determine how complex processes are organized.