Pob1 Ensures Cylindrical Cell Shape by Coupling Two Distinct Rho Signaling Events During Secretory Vesicle Targeting
Pob1 Ensures Cylindrical Cell Shape by Coupling Two Distinct Rho Signaling Events During Secretory Vesicle Targeting
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DOI：
10.1111/j.1600-0854.2011.01190.x
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发表时间：
2011-06
期刊：
影响因子：
4.5
通讯作者：
K. Nakano;M. Toya;A. Yoneda;Y. Asami;A. Yamashita;N. Kamasawa;M. Osumi;Masayuki Yamamoto
中科院分区：
文献类型：
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作者：
K. Nakano;M. Toya;A. Yoneda;Y. Asami;A. Yamashita;N. Kamasawa;M. Osumi;Masayuki Yamamoto
正确的细胞形态发生需要细胞极性、细胞骨架组织和囊泡运输的协调。粟酒裂殖酵母突变体pob 1 - 664具有奇怪的柠檬状形状，其基础尚不清楚。在这里，我们发现丰富的囊泡积累在这些细胞中，这表明Pob 1在囊泡运输中发挥作用。我们确定Rho 3作为这种表型的多拷贝抑制基因。由于Rho 3的功能与For 3（一种肌动蛋白聚合蛋白）和Sec 8（一种外囊复合物的组分）相关，我们分析了它们与Pob 1的功能关系。POB 1是必不可少的肌动蛋白电缆的形成（通过与For 3相互作用）和Sec 8的极化定位。虽然For 3和Sec 8都不是极化生长所必需的，但它们同时被破坏阻止了尖端生长，并产生了类似于pob 1 - 664的柠檬样细胞形态。因此，Pob 1可以确保S的圆柱形单元形状。通过在分泌囊泡靶向过程中偶联肌动蛋白介导的囊泡运输和外囊介导的囊泡拴系，
Proper cell morphogenesis requires the co‐ordination of cell polarity, cytoskeletal organization and vesicle trafficking. The Schizosaccharomyces pombe mutant pob1‐664 has a curious lemon‐like shape, the basis of which is not understood. Here, we found abundant vesicle accumulation in these cells, suggesting that Pob1 plays a role in vesicle trafficking. We identified Rho3 as a multicopy suppressor of this phenotype. Because Rho3 function is related to For3, an actin‐polymerizing protein, and Sec8, a component of the exocyst complex, we analyzed their functional relationship with Pob1. Pob1 was essential for the formation of actin cables (by interacting with For3) and for the polarized localization of Sec8. Although neither For3 nor Sec8 is essential for polarized growth, their simultaneous disruption prevented tip growth and yielded a lemon‐like cell morphology similar to pob1‐664. Thus, Pob1 may ensure cylindrical cell shape of S. pombe by coupling actin‐mediated vesicle transport and exocyst‐mediated vesicle tethering during secretory vesicle targeting.