Microbial Production of l-Threonine
Microbial Production of l-Threonine
复制标题
L-苏氨酸的微生物生产
DOI：
10.1271/bbb1961.33.1152
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发表时间：
1969
期刊：
影响因子：
--
通讯作者：
S. Nakamori
中科院分区：
文献类型：
--
作者：
I. Shiio;S. Nakamori
从大肠杆菌中筛选出了产l-苏氨酸的α-氨基-β-羟基戊酸抗性突变株。coliK-12，频率为3 × 10 ~（-5）。其中一株突变株β-101的L-苏氨酸产量最高，为1。9 g/升）。在来源于大肠杆菌的异亮氨酸、蛋氨酸和赖氨酸营养缺陷型中，coliK-12，只有甲硫氨酸营养缺陷型产生L-苏氨酸。相比之下，在来源于β-101的异亮氨酸、蛋氨酸和赖氨酸营养缺陷型中，L-苏氨酸积累在异亮氨酸营养缺陷型中普遍增强。其中异亮氨酸营养缺陷型菌株βI-67的L-苏氨酸产量最高，为4。7克/升）。在添加100 μg/ml L-异亮氨酸和L-甲硫氨酸的3%葡萄糖培养基中，来自β-101的甲硫氨酸营养缺陷型βM-7产生3.8 g/L，来自β1-67的甲硫氨酸营养缺陷型βIM-4产生6.1 g/L。L-苏氨酸的产率与E.大肠杆菌突变体的苏氨酸生物合成的调控机制进行了讨论。A favorable f.
l-Threonine producing α-amino-β-hydroxyvaleric acid resistant mutants were derived from E. coli K-12 with 3 x 10-5 frequency. One of mutants, strain β-101, accummulated maximum amount of l-threonine (1. 9 g/liter) in medium. Among isoleucine, methionine and lysine auxotrophs derived from E. coli K-12, only methionine auxotrophs produced l-threonine. In contrast, among isoleucine, methionine and lysine auxotrophs derived from β-101, l-threonine accumulation was generally enhanced in isoleucine auxotrophs. One of isoleucine auxotrophs, strain βI-67, produced maximum amount of l-threonine (4. 7 g/liter). Methionine auxotroph, βM-7, derived from β-101 produced 3.8 g/liter, and βIM-4, methionine auxotroph derived from β1-67, produced 6.1 g/liter, when it was cultured in 3% glucose medium supplemented with 100 μg/ml of l-isoleucine and l-methionine, respectively. These l-threonine productivities of E. coli mutants were discussed with respect to the regulatory mechanisms of threonine biosynthesis. A favourable f...