INDUCTION OF LYMPHOCYTIC LEUKEMIA, LYMPHOSARCOMA, RETICULUM-CELL SARCOMA, AND OSTEOGENIC SARCOMA IN SYRIAN GOLDEN-HAMSTER BY ONCOGENIC DNA SIMIAN VIRUS 40
INDUCTION OF LYMPHOCYTIC LEUKEMIA, LYMPHOSARCOMA, RETICULUM-CELL SARCOMA, AND OSTEOGENIC SARCOMA IN SYRIAN GOLDEN-HAMSTER BY ONCOGENIC DNA SIMIAN VIRUS 40
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DOI：
10.1093/jnci/50.5.1347
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发表时间：
1973-01-01
影响因子：
10.3
通讯作者：
DIAMANDO.GT
中科院分区：
文献类型：
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作者：
DIAMANDO.GT
用猴病毒40(SV40)静脉接种3周龄断奶雄性叙利亚金黄地鼠。在3-6个月后，85%-95%的接种病毒的动物患上了肿瘤，而所有未接种的对照组和接受SV40和抗SV40混合血清的动物都没有肿瘤疾病。诱发肿瘤有淋巴细胞性白血病、淋巴肉瘤、网状细胞肉瘤、骨肉瘤和间变性肉瘤。SV40的病原学作用的证据是间接的，通过中和病毒在体内的致癌作用，和直接，通过证明：a)在所有被测试的肿瘤的实质细胞中的SV40T(“肿瘤”)抗原，b)在原发肿瘤或肿瘤移植的成年仓鼠中产生针对SV40T抗原的抗体，c)当肿瘤细胞在敏感指示物GRET猴肾细胞的存在下生长时，肿瘤细胞在体外产生SV40病毒，以及d)在4个SV40-T阳性的淋巴肿瘤中缺乏致癌RNA病毒的血清学标记。这是除疱疹病毒组的成员外，第一次发现致癌DNA病毒可诱发淋巴细胞性白血病、淋巴肉瘤和网状细胞肉瘤。
Simian virus 40 (SV40), an oncogenic DNA virus, was inoculated intravenously into 3-week-old weanling male Syrian golden hamsters. By 3–6 months, 85–95% of the virus-inoculated animals developed neoplasms, whereas all uninoculated controls and animals that received a mixture of SV40 and anti-SV40 serum were free of neoplastic disease. The induced neoplasms were lymphocytic leukemia, lymphosarcoma, reticulum cell sarcoma, osteogenic sarcoma, and anaplastic sarcoma. Evidence for an etiologic role of SV40 was obtained both indirectly, by neutralizing the in vivo oncogenic effect of the virus by anti-SV40 serum, and directly, by demonstrating:a) SV40 T (“tumor”) antigen in parenchymal cells of all tumors tested,b) antibody production against SV40 T antigen in adult hamsters with primary tumors or tumor transplants,c) SV40 virogeny by neoplastic cells in vitro when grown in the presence of susceptible indicator grivet monkey kidney cells, andd) absence of serologic markers for oncogenic RNA viruses in 4 SV40-T-positive lymphoid neoplasms tested. This is the first time an oncogenic DNA virus, other than a member of the herpes group, has been shown to induce lymphocytic leukemia, lymphosarcoma, and reticulum cell sarcoma.