Engineering self-healing adhesive hydrogels with antioxidant properties for intrauterine adhesion prevention
Engineering self-healing adhesive hydrogels with antioxidant properties for intrauterine adhesion prevention
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DOI：
10.1016/j.bioactmat.2023.03.013
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发表时间：
2023-09-01
影响因子：
18.9
通讯作者：
Bu，Yazhong
中科院分区：
文献类型：
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作者：
Feng，Luyao;Wang，Liqun;Bu，Yazhong
宫腔内粘连（IUA）是指子宫腔内的纤维化。它是女性不孕的第二大常见原因，严重影响妇女的身心健康。目前的治疗策略未能为IUA患者提供满意的治疗结果，给生殖科学留下了巨大的挑战。具有抗氧化性能的自愈胶粘剂水凝胶对IUA的预防有很大的帮助。在这项工作中，我们制备了一系列具有抗氧化和粘接性能的自修复水凝胶（P10G15， P10G20和P10G25）。这些水凝胶具有良好的自愈性能，可以适应不同的结构。它们具有良好的注射性和适合人类子宫的形状。此外，水凝胶具有良好的组织黏附性，这对于稳定的保留和治疗效果是理想的。P10G20的体外实验表明，该黏合剂能有效清除ABTS+、DPPH和羟基自由基，使细胞免于氧化应激。此外，P10G20具有良好的血液相容性和体内外生物相容性。此外，在动物模型中，P10G20降低体内氧化应激，阻止IUA，纤维化组织减少，子宫内膜再生更好。它能有效下调纤维化相关转化生长因子β1 （TGF-β1）和血管内皮生长因子（VEGF）。总之，这些粘接剂可能是临床治疗宫内粘连的一个很好的选择。
Intrauterine adhesion (IUA) is the fibrosis within the uterine cavity. It is the second most common cause of female infertility, significantly affecting women's physical and mental health. Current treatment strategies fail to provide a satisfactory therapeutic outcome for IUA patients, leaving an enormous challenge for reproductive science. A self-healing adhesive hydrogel with antioxidant properties will be highly helpful in IUA prevention. In this work, we prepare a series of self-healing hydrogels (P10G15, P10G20, and P10G25) with antioxidant and adhesive properties. Those hydrogels exhibit good self-healing properties and can adapt themselves to different structures. They possess good injectability and fit the shape of the human uterus. Moreover, the hydrogels exhibit good tissue adhesiveness, which is desirable for stable retention and therapeutic efficacy. The in vitro experiments using P10G20 show that the adhesive effectively scavenges ABTS+, DPPH, and hydroxyl radicals, rescuing cells from oxidative stress. In addition, P10G20 offers good hemocompatibility and in vitro and in vivo biocompatibility. Furthermore, P10G20 lowers down the in vivo oxidative stress and prevents IUA with less fibrotic tissue and better endometrial regeneration in the animal model. It can effectively downregulate fibrosis-related transforming growth factor beta 1 (TGF-β1) and vascular endothelial growth factor (VEGF). Altogether, these adhesives may be a good alternative for the clinical treatment of intrauterine adhesion.