The role of vascular smooth muscle cell apoptosis and migration during uterine spiral artery remodeling in normal human pregnancy
The role of vascular smooth muscle cell apoptosis and migration during uterine spiral artery remodeling in normal human pregnancy
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DOI：
10.1096/fj.12-203679
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发表时间：
2012-07-01
期刊：
影响因子：
4.8
通讯作者：
Lash, Gendie E.
中科院分区：
文献类型：
--
作者：
Bulmer, Judith N.;Innes, Barbara A.;Lash, Gendie E.
在人子宫螺旋动脉（SpA）重塑过程中，血管平滑肌细胞（VSMCs）丢失并被纤维蛋白样细胞取代，并合并绒毛外滋养层（EVT）细胞。本研究的目的是确定SpA重塑过程中细胞凋亡和迁移对VSMC损失的相对贡献。胎盘床活检（妊娠8-20周）的免疫组织化学（Apoptag，活性半胱天冬酶3，核纤层蛋白）显示所有样品中的凋亡细胞;双重免疫标记鉴定这些为滋养层细胞，白细胞和内皮细胞。总共研究了294个SpA，仅鉴定了一个凋亡的VSMC。在部分重塑的血管中，在SpA壁周围观察到H-钙调蛋白免疫阳性的VSMC，并与之分离;与没有EVT细胞的血管相比，在有EVT细胞的血管中观察到最高水平的VSMC迁移（迁移细胞数为6.4 +/- 1.2;迁移距离为3.5 +/- 0.3像素）（迁移细胞数为3.6 +/- 0.5，P
During human uterine spiral artery (SpA) remodeling, vascular smooth muscle cells (VSMCs) are lost and replaced by fibrinoid, incorporating extravillous trophoblast (EVT) cells. The aim of the current study was to determine the relative contributions of apoptosis and migration to VSMC loss during SpA remodeling. Immunohistochemistry (Apoptag, active caspase 3, lamin) of placental bed biopsies (8-20 wk gestation) demonstrated apoptotic cells in all samples; double immunolabeling identified these as trophoblasts, leukocytes, and endothelial cells. In total, 294 SpAs were studied, and only one apoptotic VSMC was identified. H-caldesmon-immunopositive VSMCs were observed surrounding and separate from SpA walls in partially remodeled vessels; the highest level of VSMC migration was observed in vessels with associated EVT cells (number of migrated cells 6.4 +/- 1.2; distance migrated 3.5 +/- 0.3 pixels) compared with those without (number of migrated cells 3.6 +/- 0.5, P