Preparation methods for monodispersed garlic oil microspheres in water using the microemulsion technique and their potential as antimicrobials.
Preparation methods for monodispersed garlic oil microspheres in water using the microemulsion technique and their potential as antimicrobials.
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DOI：
10.1111/1750-3841.12208
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发表时间：
2013-08
影响因子：
3.9
通讯作者：
Hua Zheng;Houbin Li;Da Wei Wang;Dun Liu
中科院分区：
文献类型：
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作者：
Hua Zheng;Houbin Li;Da Wei Wang;Dun Liu
大蒜油因其广为人知的抗菌活性而被认为是一种天然的广谱抗生素。但大蒜油易挥发、水溶性差的特点限制了其在工业上的应用。本工作的目的是通过在微乳体系中负载大蒜油来研制和评价一种无油微乳。以乙氧基化氢化蓖麻(Cremvoor RH40)为表面活性剂，正丁醇(或乙醇)为助表面活性剂，油酸大蒜油为油相，超纯水为水相，制备了微乳液。考察了表面活性剂与助表面活性剂的比例和不同原油浓度对拟三元相图中水包油(O/W)微乳区的影响。考察了不同处方微乳的粒径和大蒜油包封率。此外，还测定了其对大肠杆菌和金黄色葡萄球菌的抗菌活性。实验结果表明，当表面活性剂与助表面活性剂的质量比分别为1：1、2：1、3：1时，微乳液形成稳定的微乳区。当RH40/正丁醇2/1(w/w)复配时，微乳区面积为0.089，粒径为13.29~13.85 nm，大蒜油包封率为99.5%。所制备的微乳溶液对金黄色葡萄球菌具有显著的抗菌活性。
Garlic oil is considered as a natural broad-spectrum antibiotic because of its well-known antimicrobial activity. However, the characteristics of easy volatility and poor aqueous solubility limit the application of garlic oil in industry. The purpose of the present work is to develop and evaluate an oil-free microemulsion by loading garlic oil in microemulsion system. Microemulsions were prepared with ethoxylated hydrogenated castor (Cremophor RH40) as surfactant, n-butanol (or ethanol) as cosurfactant, oleic acid-containing garlic oil as oil phase, and ultrapure water as water phase. The effects of the ratio of surfactant to cosurfactant and different oil concentration on the area of oil-in-water (O/W) microemulsion region in pseudoternary phase diagrams were investigated. The particle size and garlic oil encapsulation efficiency of the formed microemulsions with different formulations were also investigated. In addition, the antimicrobial activity in vitro against Escherichia coli and Staphylococcus aureus was assessed. The experimental results show that a stable microemulsion region can be obtained when the mass ratio of surfactant to cosurfactant is, respectively, 1:1, 2:1, and 3:1. Especially, when the mixture surfactants of RH40/n-butanol 2/1 (w/w) is used in the microemulsion formulation, the area of O/W microemulsion region is 0.089 with the particle size 13.29 to 13.85 nm and garlic oil encapsulation efficiency 99.5%. The prepared microemulsion solution exhibits remarkable antibacterial activity against S. aureus.