Glycerophospholipid profile alterations are associated with murine muscle‐wasting phenotype
Glycerophospholipid profile alterations are associated with murine muscle‐wasting phenotype
复制标题
甘油磷脂谱的改变与小鼠肌肉萎缩表型相关
DOI：
10.1002/mus.26993
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发表时间：
2020
期刊：
影响因子：
3.4
通讯作者：
Miura Shinji
中科院分区：
文献类型：
--
作者：
Senoo Nanami;Miyoshi Noriyuki;Kobayashi Eri;Morita Akihito;Tanihata Jun;Takeda Shin'ichi;Miura Shinji
磷脂是细胞膜的重要组成部分，与细胞功能密切相关，但骨骼肌磷脂谱与其生理表型的关系尚不清楚。方法我们采用液相色谱-串联质谱法进行了全面的磷脂分析，以确定来自肌肉萎缩小鼠模型的骨骼肌磷脂谱，包括失神经和Duchenne肌营养不良症小鼠模型（mdx），以及rescuedmdx小鼠表达截短dystrophin. ResultsConsistently磷脂酰胆碱和磷脂酰乙醇胺的骨骼肌中分离的变化失神经和mdx小鼠观察。值得注意的是，这些磷脂结合多不饱和脂肪酸的水平在失神经支配和mdx肌肉中减少。此外，抢救themdxpathology通过表达截短的肌营养不良蛋白导致恢复磷脂profiles.DiscussionOur研究结果支持的假设，即骨骼肌的磷脂配置文件可能与骨骼肌功能。
IntroductionPhospholipids are essential components of cellular membranes and are closely associated with cellular functions, but relationships involving skeletal muscle phospholipid profiles and their physiological phenotypes have remained unclear.MethodsWe carried out comprehensive phospholipid analyses using liquid chromatography–tandem mass spectrometry to determine the phospholipid profiles of skeletal muscles derived from muscle‐wasting mouse models, including denervated and Duchenne muscular dystrophy mouse models (mdx) as well as rescuedmdxmice expressing truncated dystrophin.ResultsConsistent phosphatidylcholine and phosphatidylethanolamine alterations in skeletal muscles isolated from denervated andmdxmice were observed. Notably, the levels of these phospholipids binding polyunsaturated fatty acids were reduced in denervated andmdxmuscles. Moreover, rescuing themdxpathology by expressing truncated dystrophin led to the restoration of phospholipid profiles.DiscussionOur findings support the hypothesis that phospholipid profiles of the skeletal muscle may be associated with skeletal muscle function.