Contribution of myeloid and lymphoid host cells to the curative outcome of mouse sarcoma treatment by photodynamic therapy
Contribution of myeloid and lymphoid host cells to the curative outcome of mouse sarcoma treatment by photodynamic therapy
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DOI：
10.1016/s0304-3835(98)00349-8
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发表时间：
1999-03-22
期刊：
影响因子：
9.7
通讯作者：
Cecic, I
中科院分区：
文献类型：
--
作者：
Korbelik, M;Cecic, I
通过选择性耗尽或灭活EMT6肉瘤小鼠的特定髓系细胞群(中性粒细胞、巨噬细胞)和淋巴系细胞群(辅助T细胞、细胞溶解T细胞)，以确定这些宿主免疫细胞类型中的每一种对光动力疗法(PDT)疗效的贡献。PDT后，中性粒细胞和溶细胞T细胞的免疫耗竭立即开始，导致PDT介导的肿瘤治愈显着减少。通过二氧化硅治疗使辅助性T细胞免疫耗尽和巨噬细胞失活，EMT6肿瘤的治愈率也显著降低。PDT最初的肿瘤消融不受上述任何一种消融治疗的影响。这些结果提供了直接证据，证明中性粒细胞、巨噬细胞和T淋巴细胞的作用对于维持对PDT治疗的肿瘤的长期控制是必不可少的。(C)1999由爱思唯尔科学有限公司出版。版权所有。
Selective depletion or inactivation of specific myeloid populations (neutrophils, macrophages) and lymphoid populations (helper T cells, cytolytic T cells) in EMT6 sarcoma-bearing mice was used to determine the contribution of each of these host immune cell types to the curative outcome of Photofrin-based photodynamic therapy (PDT). Immunodepletion of neutrophils and cytolytic T cells initiated immediately after PDT resulted in a marked reduction in PDT-mediated tumor cures. Significant reduction in the cures of EMT6 tumors was also achieved by immunodepletion of helper T cells and inactivation of macrophages by silica treatment. The initial tumor ablation by PDT was not affected by any of the above depletion treatments. These results provide direct evidence that the contribution of neutrophils, macrophages and T lymphocytes is essential for the maintenance of long-term control of PDT-treated tumors. (C) 1999 Published by Elsevier Science Ltd. All rights reserved.