Recycling of the general transcription factors during RNA polymerase II transcription.
Recycling of the general transcription factors during RNA polymerase II transcription.
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DOI：
10.1101/gad.9.12.1479
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发表时间：
1995-06
影响因子：
10.5
通讯作者：
L. Zawel;K. Kumar;D. Reinberg
中科院分区：
文献类型：
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作者：
L. Zawel;K. Kumar;D. Reinberg
我们已经分析了命运的RNA聚合酶II（RNAPII）的一般转录因子在从启动到延长使用多种方法的过渡。我们表明，所有的基础因素共存于成熟的起始复合物，但以下核苷酸添加，这个复杂的被破坏。在此转变期间，TFIID保持与启动子结合，而TFIIB、TFIIE、TFIIF和TFIIH被释放。释放后，TFIIB与TFIID重新结合，重新形成RNAPII对接位点，DB复合体。TFIIE在第十个磷酸二酯键形成之前释放。这在TFIIH释放之前，TFIIH释放发生在转录复合物达到+30之后。TFIIF的独特之处在于它是RNAPII延伸复合物中检测到的唯一基础因子。在从起始复合物释放后，TFIIF具有与停滞的RNAPII重新结合的能力。
We have analyzed the fate of the RNA polymerase II (RNAPII) general transcription factors during the transition from initiation to elongation using multiple approaches. We demonstrate that all of the basal factors coexist in mature initiation complexes but that following nucleotide addition, this complex becomes disrupted. During this transition, TFIID remains promoter-bound whereas TFIIB, TFIIE, TFIIF, and TFIIH are released. Upon release, TFIIB reassociates with TFIID, reforming the RNAPII docking site, the DB complex. TFIIE is released before formation of the tenth phosphodiester bond. This precedes TFIIH release, which occurrs after the transcription complex reaches +30. TFIIF is unique in that it is the only basal factor detected in the RNAPII elongation complex. Following its release from the initiation complex, TFIIF has the ability to reassociate with a stalled RNAPII.