Single-cell transcript analysis of pancreas development
Single-cell transcript analysis of pancreas development
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DOI：
10.1016/s1534-5807(03)00035-2
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发表时间：
2003-03-01
影响因子：
11.8
通讯作者：
Melton, DA
中科院分区：
文献类型：
--
作者：
Chiang, MK;Melton, DA
DNA微阵列分析与改良的单细胞PCR方法相结合，研究胰腺发育不同阶段单细胞的基因表达谱。该方法在胚胎第10.5天（胰腺上皮形态均匀的阶段）识别不同的细胞类型。一些细胞表达意想不到的基因组合，这些表达模式为胰腺发育提供了新的见解。根据这些发现，我们使用来自不同细胞类型的PCR产物来鉴定新的胰腺基因，其中一些标记发育中胰腺细胞的亚型。通过将这些数据与以前的遗传和生化研究相结合，我们提出了胰腺细胞发育的途径。这种形式的单细胞转录分析可以应用于任何发育过程或组织，以表征不同的细胞类型。
DNA microarray analysis was combined with a modified single-cell PCR procedure to study gene expression profiles of single cells at different stages of pancreatic development. This method identifies distinct cell types at embryonic day 10.5, a stage when the pancreatic epithelium is morphologically uniform. Some cells express unexpected combinations of genes, and these expression patterns provide new insights into pancreas development. Following on these findings, we use PCR products from different cell types to identify novel pancreatic genes, some of which mark subtypes of developing pancreatic cells. By integrating these data with previous genetic and biochemical studies, we propose a pathway for pancreatic cell development. This form of single-cell transcriptional analysis can be applied to any developmental process or tissue to characterize distinct cell types.