CRISPR/Cas9: an advanced tool for editing plant genomes
CRISPR/Cas9: an advanced tool for editing plant genomes
复制标题
DOI：
10.1007/s11248-016-9953-5
复制
发表时间：
2016-03
影响因子：
3
通讯作者：
M. Samanta;A. Dey;S. Gayen
中科院分区：
文献类型：
--
作者：
M. Samanta;A. Dey;S. Gayen
To meet current challenges in agriculture, genome editing using sequence-specific nucleases (SSNs) is a powerful tool for basic and applied plant biology research. Here, we describe the principle and application of available genome editing tools, including zinc finger nucleases (ZFNs), transcription activator-like effector nucleases (TALENs) and the clustered regularly interspaced short palindromic repeat associated CRISPR/Cas9 system. Among these SSNs, CRISPR/Cas9 is the most recently characterized and rapidly developing genome editing technology, and has been successfully utilized in a wide variety of organisms. This review specifically illustrates the power of CRISPR/Cas9 as a tool for plant genome engineering, and describes the strengths and weaknesses of the CRISPR/Cas9 technology compared to two well-established genome editing tools, ZFNs and TALENs.
