Free cottontail rabbit papillomavirus DNA persists in warts and carcinomas of infected rabbits and in cells in culture transformed with virus or viral DNA.
Free cottontail rabbit papillomavirus DNA persists in warts and carcinomas of infected rabbits and in cells in culture transformed with virus or viral DNA.
复制标题
游离的棉尾兔乳头状瘤病毒 DNA 持续存在于受感染兔子的疣和癌中以及用病毒或病毒 DNA 转化的培养物细胞中。
DOI：
10.1016/0042-6822(83)90069-7
复制
发表时间：
1983
期刊：
影响因子：
3.7
通讯作者：
Faras,AJ
中科院分区：
文献类型：
--
作者：
Watts,SL;Ostrow,RS;Phelps,WC;Prince,JT;Faras,AJ
我们比较了棉尾兔和家兔的疣和癌中棉尾兔乳头瘤病毒(CRPV)的存在和病毒DNA基因组的状态。我们的研究表明，棉尾兔的良性疣，无论是自然发现的还是在实验室诱导的，都含有大量病毒，平均每个细胞含有1000个病毒基因组副本。与棉尾兔疣相比，家兔的良性疣和癌都含有显著降低的病毒水平，平均每个细胞含有100个病毒基因组副本。一个自然产生的棉尾兔癌的单个样本每个细胞含有大约80个病毒基因组副本。通过对未消化和限制性内切酶消化的DNA样本进行Southern印迹分析，到目前为止，我们所分析的肿瘤中没有一种似乎整合了病毒基因组。此外，家兔癌和棉尾兔癌中存在的CRPV基因组通过限制性内切酶图谱显示与棉尾兔乳头状瘤和家兔乳头状瘤中的CRPV基因组相同，这表明在良性肿瘤向恶性肿瘤的发展过程中，CRPV基因组没有发生重大的缺失或重排，也不是野生型CRPV的变体导致这种现象。最后，我们展示了纯化的CRPV感染Vittop NIH 3T3和C127细胞以及转染纯化的CRPV DNA后细胞的形态变化。此外，来自转化灶的单细胞克隆含有游离形式的CRPVDNA，这种DNA在培养中持续传代。经CRPV转化的细胞在软琼脂体外生长，并在裸鼠体内产生肿瘤。
We have compared warts and carcinomas from cottontail and domestic rabbits for the presence of cottontail rabbit papillomavirus (CRPV) and the status of the viral DNA genome. Our studies indicate that benign warts from cottontail rabbits, whether found naturally or induced in the laboratory, contain large amounts of virus and on the average 1000 copies of the virus genome per cell. Both benign warts and carcinomas from domestic rabbits contain significantly reduced levels of virus relative to cottontail rabbit warts and an average of 100 copies of the virus genome per cell. A single sample of a naturally occurring cottontail rabbit carcinoma contained approximately 80 copies of the viral genome per cell. None of the tumors that we have analyzed thus far appear to have integrated viral genomes by Southern blot analysis of undigested and restriction endonuclease-digested DNA samples. Furthermore, the CRPV genome present in domestic rabbit carcinomas and a cottontail rabbit carcinoma appears identical by restriction endonuclease mapping to that present in papillomas of cottontail and domestic rabbits indicating that no major deletions or rearrangements of the CRPV genome had occurred during the progression of benign to malignant tumors nor was a variant of wild-type CRPV responsible for this phenomenon. Finally, we have demonstrated morphological transformationin vitroof NIH 3T3 and C127 cells upon infection with purified CRPV and upon transfection with purified CRPV DNA. Furthermore, single cell clones derived from transformed foci contain free forms of CRPV DNA that persist through continued passage in culture. Cells transformed by CRPV grow in soft agarin vitroand produce tumors in athymic nude mice.