Detection and characterization of quorum sensing signal molecules in Acinetobacter strains
Detection and characterization of quorum sensing signal molecules in Acinetobacter strains
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DOI：
10.1016/s0944-5013(01)80004-5
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发表时间：
2001-03-01
影响因子：
6.7
通讯作者：
Nudel, BC
中科院分区：
文献类型：
--
作者：
González, RH;Nusblat, A;Nudel, BC
群体感应是革兰氏阴性细菌中广泛存在的调节机制。在这项研究中，分析了不动杆菌菌株中是否存在能够激活 N-酰基高丝氨酸内酯生物传感器的群体感应信号分子。通过使用根癌农杆菌报告菌株，结果表明所有培养物都产生了两到四个具有不同色谱模式的可检测信号分子。在 A. calcoacetus BD413 上清液中，以时间依赖性方式检测到四种化合物，并且在稳定期达到最大活性。信号分子的数量取决于培养基的成分；通常，与复杂培养基相比，基本培养基中的培养物显示出多一个或两个信号。无论是在直接测定还是竞争测定中，不动杆菌上清液均未显示出紫色色杆菌报告菌株的自诱导活性。
Quorum sensing is a widespread regulatory mechanism among Gram-negative bacteria. In this study, Acinetobacter strains were assayed for the presence of quorum sensing signal molecules capable of activating N-acylhomoserine lactone biosensors. By using an Agrobacterium tumefaciens reporter strain it was shown that all the cultures produced two to four detectable signal molecules with different chromatographic patterns. In A. calcoaceticus BD413 supernatants four compounds were detected in a time-dependent manner, and maximal activity was reached at stationary phase. The number of signal molecules was dependent on medium composition; typically, cultures in minimal medium displayed one or two more signals, as compared to complex medium. None of the Acinetobacter supernatants showed autoinduction activity with an Chromobacterium violaceum reporter strain, neither in direct or competition assays.