metaSPAdes: a new versatile metagenomic assembler.
metaSPAdes: a new versatile metagenomic assembler.
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DOI：
10.1101/gr.213959.116
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发表时间：
2017-05
期刊：
影响因子：
7
通讯作者：
Pevzner PA
中科院分区：
文献类型：
--
作者：
Nurk S;Meleshko D;Korobeynikov A;Pevzner PA
虽然宏基因组学已成为分析细菌群体的首选技术，但宏基因组数据的组装仍然具有挑战性，从而阻碍了生物学发现。此外，最近的研究表明，复杂的细菌群体可能由数十种相关菌株组成，从而进一步放大了宏基因组组装的挑战。 metaSPAdes 通过利用已被证明在单细胞和高度多态性二倍体基因组组装中有用的计算思想来解决宏基因组组装的各种挑战。我们将 metaSPAdes 与其他最先进的宏基因组组装程序进行基准测试，并证明它可以在不同的数据集上产生高质量的组装。
While metagenomics has emerged as a technology of choice for analyzing bacterial populations, the assembly of metagenomic data remains challenging, thus stifling biological discoveries. Moreover, recent studies revealed that complex bacterial populations may be composed from dozens of related strains, thus further amplifying the challenge of metagenomic assembly. metaSPAdes addresses various challenges of metagenomic assembly by capitalizing on computational ideas that proved to be useful in assemblies of single cells and highly polymorphic diploid genomes. We benchmark metaSPAdes against other state-of-the-art metagenome assemblers and demonstrate that it results in high-quality assemblies across diverse data sets.