SEQUENCE DIVERSITY OF SMALL, ROUND-STRUCTURED VIRUSES IN THE NORWALK VIRUS GROUP
SEQUENCE DIVERSITY OF SMALL, ROUND-STRUCTURED VIRUSES IN THE NORWALK VIRUS GROUP
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DOI：
10.1128/jvi.68.9.5982-5990.1994
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发表时间：
1994-09-01
影响因子：
5.4
通讯作者：
ESTES, M
中科院分区：
文献类型：
--
作者：
WANG, JX;JIANG, X;ESTES, M
我们已经确定了一个高度保守的区域的RNA依赖的RNA聚合酶的原型雪山代理（SMA）和其他四个小的，圆形结构的病毒（抗原性诺瓦克病毒[NV]样或SMA样）从人粪便中获得的病毒RNA的逆转录-PCR扩增的核苷酸序列。粪便样本来自接受SMA给药的志愿者或1984年至1992年期间在日本和英国发生的散发病例和胃肠炎暴发。GLPSG和YGDD RNA聚合酶基序在5个SRSV的序列中处于适当的位置，但每个序列与8 FIIa原型NV序列不同，并且彼此不同。在一种新的NV抗原酶联免疫吸附试验的序列和反应性的分析表明，五种病毒可以分为两组（血清组）与NV和SMA，分别为原型。从每组的一个菌株中确定衣壳区和非结构区（2C）的序列。在日本分离的一种病毒（SRSV-KY-89/89/J）与NV原型（21年前在俄亥俄州分离）抗原相似，显示出与NV显著水平的序列相似性。KY-89和8 FIIa NV在两个开放阅读框架中的3个不同结构域中，在2,516个连续核苷酸上具有87.2%的核苷酸相似性，即96 - 98.9%的氨基酸相似性。在原型SMA和NV之间，聚合酶区域分别显示63%的核苷酸和59%的氨基酸相似性。来自英国和日本的另外两个抗原性SMA样分离株（SRSV-925/92/UK和SRSV-OTH-25/89/J）与16年和13年前在美国分离的原型SMA在聚合酶区显示出80%的核苷酸和88 - 92%的氨基酸相似性。抗原性SR SMA样OTH-25病毒的衣壳区显示与原型NV衣壳区的53%核苷酸和65%氨基酸相似性。在这两种不同的原型血清型病毒的衣壳蛋白内鉴定了序列多样性和保守性结构域。这些结果表明，NV样和SMA样因子仍在循环，序列比较将有助于鉴别和分类NV组中的不同病毒。
We have determined the nucleotide sequences of a highly conserved region of the RNA dependent RNA polymerase of the prototype Snow Mountain agent (SMA) and of four other small, round-structured viruses (antigenically Norwalk virus [NV]-like or SMA-like) following reverse transcription-PCR amplification of viral RNA obtained from human stools. The stool samples were either from volunteers administered SMA or from sporadic cases and outbreaks of gastroenteritis that occurred in Japan and the United Kingdom between 1984 and 1992. The GLPSG and YGDD RNA polymerase motifs were in the proper locations in the sequences of the five SRSVs, but each sequence was distinct from the 8FIIa prototype NV sequence and from each other. Analysis of the sequences and reactivities in a new NV antigen enzyme-linked immunosorbent assay showed that the five viruses could be divided into two groups (serogroups) with NV and SMA, respectively, being the prototypes. The sequences of the capsid region and a nonstructural region (2C) were determined from one strain from each group. One virus (SRSV-KY-89/89/J), isolated in Japan and antigenically similar to the prototype NV (isolated 21 years earlier in Ohio), showed a remarkable level of sequence similarity to NV. KY-89 and the 8FIIa NV shovved 87.2% nucleotide similarity over 2,516 continuous nucleotides amounting to 96 to 98.9% amino acid similarity in three distinct domains in two open reading frames. Between the prototype SMA and NV, the polymerase region showed 63% nucleotide and 59% amino acid similarity, respectively. Two other antigenically SMA-like isolates (SRSV-925/92/UK and SRSV-OTH-25/89/J), from the United Kingdom and Japan, showed 80% nucleotide and 88 to 92% amino acid similarity in the polymerase region to the prototype SMA isolated 16 and 13 years earlier in the United States. The capsid region of the antigenically SR SMA-like OTH-25 virus showed 53% nucleotide and 65% amino acid similarity to the prototype NV capsid region. Domains of sequence diversity and conservation were identified within the capsid protein of these two distinct prototype serotypes of virus. These results indicate that NV-like and SMA-like agents are still circulating, and sequence comparisons will be useful to identify and classify distinct viruses in the NV group.