Cardioprotective effects of the YiQiFuMai injection and isolated compounds on attenuating chronic heart failure via NF-κB inactivation and cytokine suppression
Cardioprotective effects of the YiQiFuMai injection and isolated compounds on attenuating chronic heart failure via NF-κB inactivation and cytokine suppression
复制标题
益气复脉注射液和分离化合物通过 NF-κB 失活和细胞因子抑制减轻慢性心力衰竭的心脏保护作用
DOI：
10.1016/j.jep.2013.04.019
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发表时间：
2013-06-21
影响因子：
5.4
通讯作者：
Luo, Guoan
中科院分区：
文献类型：
--
作者：
Xing, Lu;Jiang, Min;Luo, Guoan
民族药理学相关性：益气复脉注射液（YQFM）是一种治疗慢性心力衰竭的中药。本研究不仅在CHF实验模型中评估了YQFM注射液的心脏保护作用和抗炎机制，还对其体外生物活性成分进行了研究。材料与方法：结扎大鼠左冠状动脉前降支，制备CHF动物模型。由此记录大鼠心脏的心电图参数和外部征象。此外，检查心脏组织病理学，并测量炎症应激参数。实验在两个多月的时间内进行。用卡托普利治疗作为阳性对照，先前已被证明可预防CHF，未结扎lad的大鼠作为阴性对照。此外，我们通过超高效液相色谱/四极杆飞行时间质谱（UPLC/Q-TOF-MS）结合NF-kappa B活性荧光素酶报告分析系统筛选和鉴定了潜在的抗炎成分。进一步的细胞因子检测证实了YQFM中潜在的nf - κ B抑制剂的抗炎作用。结果：YQFM可显著改善CHF大鼠心功能，改善炎症介质（如肿瘤坏死因子- α、白细胞介素-6、白细胞介素-1 β）活性水平。对人参皂苷Rb1、Rg1、Rf、Rh1、Rc、Rb2、Ro和Rg3 8种潜在抗炎成分进行了表征和确认。其中，人参皂苷Ro是一种新的nf - κ B抑制剂。结论：NF-kappa B失活和细胞因子抑制可能是黄芪散对慢性心力衰竭大鼠产生改善作用的主要机制之一，人参皂苷的主要成分在治疗慢性心力衰竭中起关键作用。2013爱思唯尔爱尔兰有限公司版权所有。
Ethnopharmacological relevance: The YiQiFuMai injection (YQFM) is a traditional Chinese medicine for the treatment of chronic heart failure (CHF). The present study not only evaluated the cardioprotective effect and anti-inflammatory mechanism of the YQFM injection in an experimental model of CHF but also investigated its bioactive constituents in vitro.Materials and methods: The left anterior descending coronary artery (LAD) in rats was ligated to make an animal model of CHF. From this, electrocardiographic parameters and exterior signs of rat hearts were recorded. Additionally, the histopathology of heart tissues was examined, and parameters of inflammatory stress were measured. Experiments were performed over two months in LAD-ligation rats treated with YQFM or vehicle. Treatment with Captopril was used as a positive control, which has previously been shown to prevent CHF, and rats without LAD-ligation were used as a negative control. Furthermore, we screened and identified potential anti-inflammatory constituents by ultra-performance liquid chromatography/quadrupole time-of-flight mass spectrometry (UPLC/Q-TOF-MS) combined with NF-kappa B activity luciferase reporter assay systems. Further cytokine detection confirmed the anti-inflammatory effects of the potential NF-kappa B inhibitors from YQFM.Results: The administration of YQFM significantly improved cardiac function and ameliorated the activity level of inflammatory mediators (such as tumor necrosis factor-alpha, interleukin-6, and interleukin-1 beta) in CHF rats. Eight potential anti-inflammatory ingredients, ginsenosides Rb1, Rg1, Rf, Rh1, Rc, Rb2, Ro, and Rg3, were characterized and confirmed. Among these compounds, ginsenoside Ro was revealed as a new NF-kappa B inhibitor.Conclusion: The results suggested that NF-kappa B inactivation and cytokine suppression might be one of the main mechanisms of YQFM that caused ameliorative effects in CHF rats, and the major constituents of ginsenosides were identified playing a key role in the treatment of CHF. (C) 2013 Elsevier Ireland Ltd. All rights reserved.