INVIVO GLUCOSE-UTILIZATION IN RAT-TISSUES DURING THE 3 PHASES OF STARVATION
INVIVO GLUCOSE-UTILIZATION IN RAT-TISSUES DURING THE 3 PHASES OF STARVATION
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DOI：
10.1016/0026-0495(88)90063-7
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发表时间：
1988-11-01
影响因子：
9.8
通讯作者：
LEMAHO, Y
中科院分区：
文献类型：
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作者：
CHEREL, Y;BURNOL, AF;LEMAHO, Y
根据鸟类和哺乳动物蛋白质和脂质利用的变化，描述了饥饿的三个阶段。在本研究中，在这三个阶段，在体内组织葡萄糖利用率进行了测量，使用2-脱氧-[1- 3 H]葡萄糖技术在麻醉大鼠。因此，根据这项技术，术语葡萄糖利用是指葡萄糖在组织中的运输和磷酸化，即葡萄糖的命运。全身葡萄糖周转率，这是由连续输注的[3- 3 H]葡萄糖，减少了40%，在前两天的饥饿（阶段1），它没有改变，此后，无论是在蛋白质节约阶段2，也不是在阶段3，这是由净蛋白质分解的增加为标志。两天的饥饿引起骨骼肌中的葡萄糖利用率显着下降，这种下降是在氧化肌肉（65%，在膈肌，66%，比目鱼肌）比糖酵解肌肉（31%，趾长伸肌，34%，上滑车）。心房（75%）、心室（93%）和白色脂肪组织（54%）的葡萄糖利用率也降低;相比之下，棕色脂肪组织的葡萄糖利用率增加了两倍，大脑和皮肤没有变化。从第1阶段到第2阶段，在任何组织中均未观察到葡萄糖利用的变化。然而，第3阶段的特点是趾长伸肌（45%）、棕色脂肪组织（76%）、大脑（29%）和皮肤（40%）的葡萄糖利用率降低，而膈肌和心室的葡萄糖利用率分别增加了2.3倍和3.4倍。我们的结论是，长期饥饿不会改变全身葡萄糖节约适应，即使在低可用性的脂质燃料的情况下（第3阶段）。尽管如此，在这个阶段，各个组织之间的葡萄糖利用会以有利于工作肌肉的方式进行重新排列。
Three phases of starvation have been described from changes in protein and lipid utilization in birds and mammals. In the present study, tissue glucose utilization was measured in vivo during these three phases, using a 2-deoxy-[1-3H]glucose technique in the anesthetized rat. According to this technique, the term glucose utilization therefore refers to transport and phosphorylation of glucose in tissues, ie, whatever is the fate of glucose. Whole-body glucose turnover rate, which was determined by a continuous infusion of [3-3H]glucose, decreased by 40% during the first two days of starvation (phase 1); it did not change thereafter, neither in the protein-sparing phase 2 nor in phase 3, which is marked by an increase in net protein breakdown. Two days of starvation caused a marked decrease in the glucose utilization in skeletal muscles; this decrease was higher in oxidative muscles (65% in diaphragm, 66% in soleus) than in glycolytic muscles (31% in extensor digitorum longus, 34% in epitrochlearis). Glucose utilization also decreased in heart atria (75%), heart ventricles (93%), and white adipose tissue (54%); by contrast, there was a two-fold increase in glucose utilization in brown adipose tissue and no change in brain and skin. No variations were observed in glucose utilization in any of the tissues from phase 1 to phase 2. However, phase 3 was marked by a decrease in glucose utilization in extensor digitorum longus (45%), brown adipose tissue (76%), brain (29%), and skin (40%), whereas there was a 2.3- and 3.4-fold increase in glucose utilization in diaphragm and heart ventricles, respectively. We conclude that prolonged starvation does not alter the whole-body glucose-sparing adaptation, even in the situation of low availability in lipid fuels (phase 3). Nevertheless, there is at this stage a rearrangement in glucose utilization among individual tissues in a way that favors the working muscles.