Biophotography: concepts, applications and perspectives
Biophotography: concepts, applications and perspectives
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DOI：
10.1007/s00253-016-7384-0
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发表时间：
2016-02
影响因子：
5
通讯作者：
Christian Renicke;Christof Taxis
中科院分区：
文献类型：
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作者：
Christian Renicke;Christof Taxis
合成生物学旨在通过合理设计和遗传引入的成分来操纵生物系统。光致动器工程的努力导致微生物对细胞外光信号产生各种细胞反应。其中一些导致形成宏观可观察的输出，可用于生成由生命物质构成的图像。已经开发了几种通过酶促转化将无色化合物转化为可见色素的方法。这已被利用作为成功创建光遗传学工具的展示;已与此生物摄影相结合的基本光控生物过程的例子包括转录，蛋白质稳定性和第二信使合成的调节。此外，图像的生物再现被用作促进光遗传学开关的定量表征的手段以及研究复杂的细胞信号传导电路的技术。在这里，我们将比较不同的生物图像生成技术，介绍实验方法，并提供生物摄影的未来前景。
Synthetic biology aims at manipulating biological systems by rationally designed and genetically introduced components. Efforts in photoactuator engineering resulted in microorganisms reacting to extracellular light-cues with various cellular responses. Some of them lead to the formation of macroscopically observable outputs, which can be used to generate images made of living matter. Several methods have been developed to convert colorless compounds into visible pigments by an enzymatic conversion. This has been exploited as a showcase for successful creation of an optogenetic tool; examples for basic light-controlled biological processes that have been coupled to this biophotography comprise regulation of transcription, protein stability, and second messenger synthesis. Moreover, biological reproduction of images is used as means to facilitate quantitative characterization of optogenetic switches as well as a technique to investigate complex cellular signaling circuits. Here, we will compare the different techniques for biological image generation, introduce experimental approaches, and provide future-perspectives for biophotography.