PHARMACOLOGICAL PROPERTIES OF SOME CRUSTACEAN NEURONAL ACETYLCHOLINE, GAMMA-AMINOBUTYRIC ACID, AND L-GLUTAMATE RESPONSES
PHARMACOLOGICAL PROPERTIES OF SOME CRUSTACEAN NEURONAL ACETYLCHOLINE, GAMMA-AMINOBUTYRIC ACID, AND L-GLUTAMATE RESPONSES
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DOI：
10.1113/jphysiol.1978.sp012381
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发表时间：
1978-01-01
影响因子：
5.5
通讯作者：
PAUPARDINTRITSCH, D
中科院分区：
文献类型：
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作者：
MARDER, E;PAUPARDINTRITSCH, D
用L-谷氨酸、γ-氨基丁酸和乙酰胆碱(ACh)对中华绒蟹口胃神经节细胞的反应进行了研究。离子电泳法或加压L谷氨酸可引起3种反应：钾离子依赖性抑制反应，在15-20 mV时逆转，负值高于静息电位；氯离子依赖性抑制反应，在静息电位处于平衡状态；去极化反应。离子导入或加压GABA同样产生3种反应：K+电导增加，Cl-电导增加和去极化反应。印防己毒素(10-6~10-5M)能有效阻断谷氨酸抑制反应。10~(-4)M-印防己毒素对GABA-Cl~-反应无影响，可阻断50%的GABA-K~+依赖反应。β-鸟嘌呤丙酸(β-GP)是GABA-K+反应的激动剂，但对模拟或阻断GABA-Cl-反应无效。ACh的应用产生了巨大的去极化反应，其药理特征类似于脊椎动物的尼古丁神经节反应。M受体激动剂乙酰-β-甲基胆碱(MAECH)产生去极化，其幅度随膜超极化从-40 mV降至-100 mV而降低。匹罗卡品和氧托莫林可引起神经节细胞内源性活动的改变。这些结果对确定口胃神经节中的突触递质的意义进行了讨论。
L-glutamate, GABA, and acetylcholine (ACh) responses of cells in the stomatogastric ganglion of the crab, Cancer pagurus were studied. Ionophoretic or pressure application of L-glutamate revealed 3 classes of responses: a K+-dependent inhibition which reversed at 15-20 mV more negative than the resting potential; a Cl- dependent inhibitory response which was at equilibrium at the resting potential; and a depolarizing response. Ionophoretic or pressure applications of GABA likewise produced 3 kinds of responses: an increase in K+ conductance, an increase in Cl- conductance and a depolarizing response. Picrotoxin (10-6-10-5 M) was effective in blocking both the glutamate inhibitory responses. 10-4 M-picrotoxin, necessary to produce a 50% block of the GABA-K+-dependent response, had no effect on the GABA-Cl- response. .beta.-Guanidinopropionic acid (.beta.-GP) was an agonist for the GABA-K+ response, but was ineffective in mimicking or blocking the GABA-Cl- response. ACh applications produced large depolarizing responses with a pharmacological profile similar to that of the nicotinic ganglionic response in vertebrates. The muscarinic agonist, acetyl-.beta.-methyl choline (MeCh), produced depolarizations which decreased in amplitude as the membrane was hyperpolarized from -40 to -100 mV. Pilocarpine and oxotremorine produced changes in the endogenous activity of ganglionic neurons. Implications of these results for identification of synaptic transmitters in the stomatogastric ganglion are discussed.