Simultaneous determination of nine saponins from Panax notoginseng using HPLC and pressurized liquid extraction
Simultaneous determination of nine saponins from Panax notoginseng using HPLC and pressurized liquid extraction
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DOI：
10.1016/j.jpba.2005.10.023
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发表时间：
2006-04-11
影响因子：
3.4
通讯作者：
Wang, YT
中科院分区：
文献类型：
--
作者：
Wan, JB;Lai, CM;Wang, YT
建立了同时测定三七中三七皂苷R1、人参皂苷Rg1、Re、Rf Rb1、Rc、Rb2、Rb3和Rd 9种皂苷含量的高效液相色谱法和加压液萃取法。采用C-18色谱柱，水-乙腈梯度洗脱，通过与参比化合物的保留时间、质谱等进行鉴定。采用PLE法、超声法、索氏提取法和浸渍法制备样品，并对其提取效率进行了比较。结果表明，该方法具有较高的提取效率和重复性，可为中药质量控制的标准制样提供参考。建立的高效液相色谱法和液相色谱法是同时定量测定三七中皂苷含量的有效方法，可用于三七及其制剂的质量控制。(c) 2005 Elsevier B.V.版权所有
A HPLC and pressurized liquid extraction (PLE) method was developed for simultaneous determination of nine saponins, including notoginsenoside R1, ginsenoside Rg1, Re, Rf Rb1, Rc, Rb2, Rb3 and Rd in Panax notoginseng. The analysis was performed on C-18 column with water-acetonitrile gradient elution and the investigated saponins were authenticated by comparing, retention time and mass spectra with their reference compounds. Several methods including PLE, ultrasonication, soxhlet extraction and immersion were used for sample preparation and their extraction efficiency was compared. The results showed that PLE has the highest extraction efficiency and repeatability, which would be valuable on standardization of sample preparation for quality control of Chinese medicines. The developed HPLC and PLE is an effective approach for simultaneously quantitative determination of sapoinins in P. notoginseng, which could be used for quality control of P. notoginseng and its preparations. (c) 2005 Elsevier B.V. All rights reserved.