Host species-specific usage of the TLR4-LPS receptor complex
Host species-specific usage of the TLR4-LPS receptor complex
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DOI：
10.1177/1753425908095957
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发表时间：
2008-08-01
期刊：
影响因子：
3.2
通讯作者：
Werling, Dirk
中科院分区：
文献类型：
--
作者：
Lizundia, Reoina;Sauter, Kav-Sara;Werling, Dirk
LIPS 的识别取决于形成 LPS-受体复合物的至少三个分子的相互作用。最重要的是，CD14。 MD2 和 Toll 样受体 (TLR) 4 在物种之间具有高度的同源性。在本研究中，我们使用稳定转染的表达人或牛 LPS 受体复合物成分的 HEK293 细胞系，研究了物种特异性限制对 LPS 识别的重要性。物种特异性 MD2 似乎赋予 LPS 识别，而物种特异性 CD14 似乎仅发挥次要作用。除了LPS的认可。有证据表明，呼吸道合胞病毒 (RSV) 是全球婴儿期最常见的病毒性呼吸道病原体，其融合 (F) 蛋白与 TLR4 相互作用，并在先天免疫反应的启动中发挥重要作用。我们的研究结果表明，人和牛 RSV 可能会激活人和牛 TLR4 受体。分别在 MD2 和 CD14 存在的情况下。然而，没有观察到人或牛 RSV 的 RSV IF 蛋白单独刺激 TLR4 依赖性 NF-κ B 激活的明确作用。
Recognition of LIPS depends on the interaction of at least three molecules forming the LPS-receptor complex. The Most important ones, CD14. MD2 and Toll-like receptor (TLR) 4 share a high degree of homology between species. In the present study, we investigated file importance of species-specific restriction on the recognition of LPS using stably transfected HEK293 cell lines expressing either human or bovine LPS-receptor complex components. Species-specific MD2 appeared to confer LPS recognition, whereas species-specific CD14 only appeared to play a minor role. In addition to the recognition of LPS. there is evidence that the fusion (F) protein of respiratory syncytial virus (RSV), which is the most common viral respiratory pathogen during infancy world-wide, interacts with TLR4, and plays an important role in the initiation of the innate immune response. Our findings suggest that human and bovine RSV may,activate human and bovine TLR4 receptors. respectively, in the presence of both MD2 and CD14. However, no clear role for the RSV IF protein of either human or bovine RSV alone in stimulating TLR4-dependent NF-kappa B activation was observed.