Multigene phylogeny of root-knot nematodes and molecular characterization of Meloidogyne nataliei Golden, Rose & Bird, 1981 (Nematoda:Tylenchida)
Multigene phylogeny of root-knot nematodes and molecular characterization of Meloidogyne nataliei Golden, Rose & Bird, 1981 (Nematoda:Tylenchida)
复制标题
DOI：
10.1038/s41598-019-48195-0
复制
发表时间：
2019-08-13
影响因子：
4.6
通讯作者：
Subbotin, Sergei A.
中科院分区：
文献类型：
--
作者：
Alvarez-Ortega, Sergio;Brito, Janete A.;Subbotin, Sergei A.
根结线虫属（Meloidogyne）植物根结线虫是一种高度适应性的专性植物寄生线虫，约有100个有效种，是植物寄生线虫中最重要的经济类群。6种根结线虫：M. arenaria，M.哈普拉，M。incognita，M. microtyla，M. naasi和M. nataliei之前在美国密歇根州报道过。在这项研究中，从美国密歇根州的模式产地的葡萄藤葡萄属labrusca中分离出纳氏根结线虫，并使用同工酶分析和核糖体和线粒体进行表征！基因序列用1、5、6、7或10只雌鼠的浸渍液未检测到苹果酸脱氢酶活性。nataliei per well.然而，一个强带（EST= 51; Rm：27.4）的酯酶活性检测时，每孔使用10个产卵的女性的匀浆。对55种有效根结线虫的18 S核糖体RNA部分序列、285 rRNA的D2-D3序列、rRNA的内转录间隔区序列、线粒体细胞色素氧化酶亚基I基因和细胞色素氧化酶亚基II-165 rRNA基因的属间序列进行了系统发育分析。使用贝叶斯推断和最大似然方法进行。从这些结果中，我们推断出11个不同的分支研究种，M。nataliei和M.组成一个基本谱系的印度种。这些物种中的75%属于根结线虫超级进化枝内的7个进化枝。这些分支的特点和进化趋势内的根结线虫进行了讨论。
The root-knot nematodes of the genus Meloidogyne are highly adapted, obligate plant parasites, consisting of nearly one hundred valid species, and are considered the most economically important group of plant-parasitic nematodes. Six Meloidogyne species: M. arenaria, M. hapla, M. incognita, M. microtyla, M. naasi and M. nataliei were previously reported in Michigan, USA. For this study, Meloidogyne nataliei was isolated from the grapevine Vitis labrusca from the type locality in Michigan, USA, and was characterized using isozyme analysis and ribosomal and mitochondria! gene sequences. No malate dehydrogenase activity was detected using macerate of one, five, six, seven or ten females of M. nataliei per well. However, one strong band (EST= 51; Rm: 27.4) of esterase activity was detected when using homogenates of ten egg-laying females per well. Phylogenetic analyses of sequences of the partial 18S ribosomal RNA, D2-D3 of 285 rRNA, internal transcribed spacer of rRNA, mitochondrial cytochrome oxidase subunit I genes and the cytochrome oxidase subunit II-165 rRNA intergeneric fragment from fifty-five valid Meloidogyne species and M. nataliei were conducted using Bayesian inference and maximum likelihood methods. From these results, we infer 11 distinct clades among studied species, with M. nataliei and M. indica composing a basal lineage. Seventy five percent of these species belong to seven clades within the Meloidogyne superclade. Characterization of these clades is provided and evolutionary trends within the root-knot nematodes are discussed.