Human amniotic epithelial cells are promising transgene carriers for allogeneic cell transplantation into liver
Human amniotic epithelial cells are promising transgene carriers for allogeneic cell transplantation into liver
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DOI：
10.1007/s100380050205
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发表时间：
2000-05
影响因子：
3.5
通讯作者：
N. Sakuragawa;S. Enosawa;T. Ishii;R. Thangavel;T. Tashiro;T. Okuyama;Seiichi Suzuki
中科院分区：
文献类型：
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作者：
N. Sakuragawa;S. Enosawa;T. Ishii;R. Thangavel;T. Tashiro;T. Okuyama;Seiichi Suzuki
由于人羊膜上皮组织是在受精后第8天左右形成的，人羊膜上皮细胞(HAEC)可能具有分化为各种器官的多潜能，如脑、心脏或肝脏。在这项研究中，我们通过免疫染色和酶联免疫分析证明了hAEC合成和排泄白蛋白的证据。逆转录-聚合酶链式反应(RT-PCR)和免疫印迹分析分别检测到白蛋白mRNA和蛋白的表达。此外，hAEC对人血清白蛋白、α胎蛋白等肝系遗传标志物也有免疫反应。移植到SCID小鼠肝脏的人AEC对白蛋白和α-胎儿蛋白呈阳性免疫反应。将含有β-半乳糖苷酶基因的转基因细胞(AxCALacZ)整合到肝实质中。对植入hAEC后的SCID小鼠肝脏进行的人类多态基因分析表明，直到细胞移植后第7天，这些SCID小鼠的肝脏都明显含有这种人类特有的基因。由于hAEC不会引起同种异体移植的急性排斥反应，我们认为hAEC可以作为转基因载体用于遗传性肝病患者的治疗。
As human amniotic epithelial tissue is formed on about the eighth day after fertilization, human amniotic epithelial cells (hAEC) may have multipotency to differentiate into various organs, such as brain, heart, or liver. In this study, we showed evidence of the synthesis and excretion of albumin by hAEC, by immunostaining and enzyme-linked immunoassay. Reverse transcription-polymerase chain reaction (RT-PCR) and western blot analyses revealed the expression of albumin mRNA and protein, respectively. In addition, hAEC also demonstrated immunoreactivity to genetic markers of liver lineage, such as human serum albumin and α-fetoprotein. Transplanted hAEC to Scid mouse liver showed positive immunoreactivity to albumin and α-fetoprotein. Genetically modified cells containing the β-galactosidase (LacZ) gene (AxCALacZ) were integrated in liver parenchyma. Human polymorphic gene analysis in Scid mouse liver after the implantation of hAEC showed that these Scid mouse livers obviously contained this human-specific gene until day 7 after the cell transplantation. As hAEC do not cause any acute rejection by allotransplantation, we conclude that hAEC may be useful as a transgene carrier to treat patients with inherited liver diseases.