Nisin adsorption to polyethylene oxide layers and its resistance to elution in the presence of fibrinogen.
Nisin adsorption to polyethylene oxide layers and its resistance to elution in the presence of fibrinogen.
复制标题
乳链菌肽对聚环氧乙烷层的吸附及其在纤维蛋白原存在下的耐洗脱性。
DOI：
10.1016/j.jcis.2010.06.038
复制
发表时间：
2010
影响因子：
9.9
通讯作者：
Neff,JenniferA
中科院分区：
文献类型：
--
作者：
Ryder,MatthewP;Schilke,KarlF;Auxier,JulieA;McGuire,Joseph;Neff,JenniferA
吸附和洗脱的抗菌肽乳酸链球菌素在硅烷化的二氧化硅表面涂覆到本悬垂聚氧化乙烯链原位检测zeta电位测量。用三氯乙烯基硅烷处理二氧化硅微球以引入疏水性乙烯基基团，然后自组装聚环氧乙烷-聚环氧丙烷-聚环氧乙烷（PEO-PPO-PEO）三嵌段表面活性剂Pluronic® F108或具有次氮基三乙酸端基的F108衍生物。对三嵌段包覆的微球进行γ辐照以共价稳定PPO-表面缔合。PEO层的稳定性通过对SDS洗脱的三嵌段抗性来评价，并且层的均匀性通过纤维蛋白原排斥来评价。将乳链菌肽引入未涂覆的或三嵌段涂覆的微球中产生了作为阳离子肽吸附的结果的表面电荷（zeta电位）的显著正变化。在连续的吸附实验中，纤维蛋白原的乳酸链球菌素加载的三嵌段层的引入引起zeta电位的降低，这与乳酸链球菌素的部分洗脱和/或纤维蛋白原在界面处的优先位置是一致的。这种变化对于未涂覆的二氧化硅比三嵌段涂覆的二氧化硅明显得多，表明PEO层提供了增强的对乳链菌肽洗脱的抗性。
The adsorption and elution of the antimicrobial peptide nisin at silanized silica surfaces coated to present pendant polyethylene oxide chains was detected in situ by zeta potential measurements. Silica microspheres were treated with trichlorovinylsilane to introduce hydrophobic vinyl groups, followed by self assembly of the polyethylene oxide–polypropylene oxide–polyethylene oxide (PEO–PPO–PEO) triblock surfactant Pluronic® F108, or an F108 derivative with nitrilotriacetic acid end groups. Triblock-coated microspheres were γ-irradiated to covalently stabilize the PPO-surface association. PEO layer stability was evaluated by triblock resistance to elution by SDS, and layer uniformity was evaluated by fibrinogen repulsion. Introduction of nisin to uncoated or triblock-coated microspheres produced a significant positive change in surface charge (zeta potential) as a result of adsorption of the cationic peptide. In sequential adsorption experiments, the introduction of fibrinogen to nisin-loaded triblock layers caused a decrease in zeta potential that was consistent with partial elution of nisin and/or preferential location of fibrinogen at the interface. This change was substantially more pronounced for uncoated than triblock-coated silica, indicating that the PEO layer offers enhanced resistance to nisin elution.