Including fluorescent nanoparticle probes within injectable gels for remote strain measurements and discrimination between compression and tension.
Including fluorescent nanoparticle probes within injectable gels for remote strain measurements and discrimination between compression and tension.
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DOI：
10.1039/d0sm01635g
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发表时间：
2020-12
期刊：
影响因子：
3.4
通讯作者：
Hannah R Shanks;Shangli Wu;N. T. Nguyen;Dongdong Lu;B. Saunders
中科院分区：
文献类型：
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作者：
Hannah R Shanks;Shangli Wu;N. T. Nguyen;Dongdong Lu;B. Saunders
远程和非侵入性地监测和测量用于增强软组织的可注射凝胶内的应变的能力是非常理想的。这些信息可以实时监测凝胶性能和定制凝胶设计。我们报告了这一目标的进展，使用包括在两个不同的注射凝胶中的两个荧光粒子探针系统。这两种可注射凝胶之前已经在椎间盘修复和软骨修复的可伸展凝胶的背景下进行了研究。这两个荧光粒子探测器发射的是蓝色或近红外(NIR)，浓度非常低。蓝色发射探头的归一化光致发光(PL)强度等于凝胶的压缩变形比。此外，蓝色和近红外探测器的光致发光强度的归一化比在很大范围内(从0.2到3.0)随变形比呈线性变化，从压缩到拉伸的无缝过渡。因此，PL可以区分压缩和拉伸。这里建立的新方法应该适用于其他凝胶，并能够远程检测凝胶是否被压缩或拉伸以及程度。这项研究可能为远程和微创地测量体内负荷支持凝胶所经历的应变提供了重要的一步。
The ability to remotely and non-invasively monitor and measure the strain within injectable gels used to augment soft tissue is highly desirable. Such information could enable real-time monitoring of gel performance and bespoke gel design. We report progress towards this goal using two fluorescent particle probe systems included within two different injectable gels. The two injectable gels have been previously studied in the contexts of intervertebral disc repair and stretchable gels for cartilage repair. The two fluorophore particle probes are blue or near-infrared (NIR) emitting and are present at very low concentrations. The normalised photoluminescence (PL) intensity from the blue emitting probe is shown to equal the compressive deformation ratio of the gels. Furthermore, the normalised ratio of the PL intensities for the blue and NIR probes varies linearly with deformation ratio over a wide range (from 0.2 to 3.0) with a seamless transition from compression to tension. Hence, PL can discriminate between compression and tension. The new approach established here should apply to other gels and enable remote detection of whether a gel is being compressed or stretched as well as the extent. This study may provide an important step towards remotely and minimally invasively measuring the strain experienced by load-supporting gels in vivo.