Acute and chronic effects of ethanol on transbilayer membrane domains.
Acute and chronic effects of ethanol on transbilayer membrane domains.
复制标题
乙醇对跨双层膜域的急性和慢性影响。
DOI：
10.1111/j.1471-4159.1989.tb07278.x
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发表时间：
1989
影响因子：
4.7
通讯作者：
Schroeder,F
中科院分区：
文献类型：
--
作者：
Wood,WG;Gorka,C;Schroeder,F
醇类，包括乙醇，对跨双层和侧膜结构域有特定的影响。最近的证据表明，体外酒精对一个瓣叶的流动性的影响大于另一个瓣叶。本研究采用三硝基苯磺酸（TNBS）标记和1，6-二苯基-1，3，5-己三烯（DPH）的微分偏振荧光法，研究了慢性乙醇消耗对突触质膜（SPM）外表面和细胞面小叶流动性的影响。小鼠被给予乙醇或对照液体饮食3周。处死动物并制备SPM。在两组中，SPM的外表面瓣叶比细胞面瓣叶的流体性显著更高，如DPH的限制性各向异性所示。然而，乙醇处理组中两个瓣叶之间的差异要小得多。临床上观察到的浓度的乙醇在体外对流体性更强的外表面小叶有更大的影响。乙醇的这种不对称效应在乙醇处理的小鼠的外面部小叶中显著减弱。慢性乙醇消耗对膜有特定的影响。膜的功能，可能是由不对称的流动性和脂质分布的调节可能会改变慢性乙醇消耗。
Alcohols, including ethanol, have a specific effect on transbilayer and lateral membrane domains. Recent evidence has shown that alcohols in vitro have a greater effect on fluidity of one leaflet as compared to the other. The present study examined effects of chronic ethanol consumption on fluidity of synaptic plasma membrane (SPM) exofacial and cytofacial leaflets using trinitrobenzenesulfonic acid (TNBS) labeling and differential polarized fluorometry of 1,6‐diphenyl‐1,3,5‐hexatriene (DPH). Mice were administered ethanol or a control liquid diet for 3 weeks. Animals were killed and SPM prepared. The exofacial leaflet of SPM was significantly more fluid than the cytofacial leaflet in both groups, as indicated by limiting anisotropy of DPH. However, differences between the two leaflets were much smaller in the ethanol‐treated group. Ethanol at concentrations seen clinically had a greater effect in vitro on the more fluid exofacial leaflet. This asymmetric effect of ethanol was significantly diminished in the exofacial leaflet of the ethanol‐treated mice. Chronic ethanol consumption has a specific effect on membranes. Membrane functions that may be regulated by asymmetry of fluidity and lipid distribution may be altered by chronic ethanol consumption.