BIOCHEMICALLY ACTIVE SOL-GEL GLASSES - THE TRAPPING OF ENZYMES
BIOCHEMICALLY ACTIVE SOL-GEL GLASSES - THE TRAPPING OF ENZYMES
复制标题
DOI：
10.1016/0167-577x(90)90002-4
复制
发表时间：
1990-09-01
影响因子：
3
通讯作者：
OTTOLENGHI, M
中科院分区：
文献类型：
--
作者：
BRAUN, S;RAPPOPORT, S;OTTOLENGHI, M
我们描述了一种生物化学活性的溶胶-凝胶玻璃的制备和性能，该玻璃是通过在聚合四甲基氧基硅烷中捕获碱性磷酸酶（ALP）而获得的。与溶液相比，固定化纯化的牛肠黏膜ALP具有30%的活性产率和更好的热失活稳定性。复合生物活性玻璃在室温下在水中保存两个月而不失去活性。观察到非michaelis - menten动力学。用溶胶-凝胶法制备生物活性材料的概念似乎是通用的。因此，其他酶（几丁质酶、天冬氨酸酶、β-葡萄糖苷酶）被成功捕获。
We describe the preparation and properties of a biochemically active sol-gel glass, obtained by trapping the enzyme alkaline phosphatase (ALP) in a polymerizing tetramethoxysilane. The immobilized purified ALP from bovine intestinal mucosa had a 30% activity yield and an improved stability to thermal deactivation compared to a solution. The composite bioactive glass was preserved in water at room temperature for two months without loosing activity. A non-Michaelis-Menten kinetics was observed. The concept of preparing bioactive materials by the sol-gel method seems to be general. Thus, other enzymes (chitinase, aspartase, β-glucosidase) were successfully trapped.