CD8+ T cells responding to influenza infection reach and persist at higher numbers than CD4+ T cells independently of precursor frequency
CD8+ T cells responding to influenza infection reach and persist at higher numbers than CD4+ T cells independently of precursor frequency
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DOI：
10.1016/j.clim.2004.05.006
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发表时间：
2004-10-01
影响因子：
8.6
通讯作者：
Dutton, RW
中科院分区：
文献类型：
--
作者：
Powell, TJ;Brown, DM;Dutton, RW
研究了流感感染后CFSE标记的初始流感特异性CD8(+)和CD4(+) T细胞的活化、定位、表型变化和功能。过继转移的CD8(+) T细胞的反应最早在引流淋巴结中被观察到。后来在肺、气道和脾脏中发现高度活化的细胞，这些细胞具有细胞溶解作用，并且在再次刺激时表达干扰素 -γ。在感染前同时转移CD4(+)和CD8(+) T细胞然后感染，在感染后9天和30天检测到早期时间点的分裂数量相似，但CD8(+) T细胞数量更多。转移数量少得多的CD4(+)和CD8(+) T细胞导致更广泛的扩增，但两种细胞类型最终数量的差异相同。这些研究表明了CD8(+)和CD4(+) T细胞在感染后的早期时间点如何对流感作出反应，以及抗原特异性CD4(+)和CD8(+) T细胞的动力学差异。(C) 2004爱思唯尔公司。保留所有权利。
The activation, localization, phenotypic changes, and function of CFSE-labeled naive influenza-specific CD8(+) and CD4(+) T cells following influenza infection were examined. Response of adoptively transferred CD8(+) T cells was seen earliest in draining lymph node. Highly activated cells were found later in the lung, airways, and spleen, were cytolytic, and expressed IFN-gamma upon restimulation. Similar amounts of division at early time points, but higher numbers of CD8(+) T cells, were detected at 9 and 30 days postinfection after cotransfer of CD4(+) and CD8(+) T cells followed by infection. Transfer of much smaller numbers of CD4(+) and CD8(+) T cells led to more extensive expansion but the same difference in final number between the two cell types. These studies demonstrate how CD8(+) and CD4(+) T cells respond to influenza at early time points postinfection and the differential kinetics of antigen-specific CD4(+) and CD8(+) T cells. (C) 2004 Elsevier Inc. All rights reserved.