Effects of "wettability" of biomaterials on culture cells.
Effects of "wettability" of biomaterials on culture cells.
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生物材料“润湿性”对培养细胞的影响。
DOI：
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发表时间：
1989
影响因子：
1.6
通讯作者：
E. Sairenji
中科院分区：
文献类型：
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作者：
I. Yanagisawa;H. Sakuma;M. Shimura;Y. Wakamatsu;S. Yanagisawa;E. Sairenji
近年来生物材料发展的新目标包括如何控制材料的表面特性和细胞在植入部位的附着。这项研究阐明了材料的“润湿性”对培养细胞的影响，润湿性用材料与水的接触角来表示。首先，进行低温等离子体处理，以获得相同材料和形状的样品，只是润湿性不同。然后测量了样品表面的接触角和表面粗糙度，并用ESCA对样品表面进行了分析。为了阐明细胞对润湿性的生物学反应，在每个测试样本中都研究了结缔组织和上皮组织起源的已建立的细胞系与材料的附着及其细胞扩散。结果发现，实验中使用的每种材料的接触角都影响细胞的附着和铺展速度，因此，生物材料的润湿性被认为是细胞水平上生物效应的一个重要参数。
New objectives of the development of biomaterials in recent years include how to control surface characteristics of materials and the attachment of cells to implant sites. This study clarified the effect of "wettability" of materials on culture cells, with wettability being expressed by the contact angle of the material to the water. First, low-temperature plasma treatment was administered so that samples of the same materials and shapes could be obtained, differing only in wettability. The contact angles at the surfaces of the samples and their surface roughness were then measured, and surfaces were analyzed by ESCA. For clarification of the biological response of the cell to wettability, attachment of connective tissues and epithelial-tissue-originated established cell lines to the material and its cell spreading were investigated in each test sample. As a result, it was found that the contact angle of each material used in the experiment affected both the cell attachment and spreading rates; thus, wettability of biomaterials is considered to be an important parameter of biological effect at the cell level.