Gelatin nanospheres incorporating siRNA for controlled intracellular release
Gelatin nanospheres incorporating siRNA for controlled intracellular release
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DOI：
10.1016/j.biomaterials.2012.08.032
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发表时间：
2012-12-01
期刊：
影响因子：
14
通讯作者：
Tabata, Yasuhiko
中科院分区：
文献类型：
--
作者：
Ishikawa, Hidefumi;Nakamura, Yoko;Tabata, Yasuhiko
本研究的目的是开发一种细胞内小干扰RNA（siRNA）的持续释放系统，旨在延长基因抑制的时间。将含有荧光素酶siRNA的明胶水溶液通过加入丙酮进行共溶，然后通过戊二醛（GA）交联明胶来制备包含siRNA的明胶纳米球。纳米球在含有胶原酶的磷酸盐缓冲盐水溶液中随时间降解以释放掺入的siRNA。随着GA浓度的增加，纳米球降解速度变慢，因此siRNA的速率变低。由于纳米球降解，siRNA从纳米球释放。将纳米球内化到稳定表达荧光素酶的结肠26细胞中，而与GA浓度无关。通过掺入能够长期释放的siRNA的纳米球抑制基因表达，随后延长基因抑制的时间段。观察到细胞内的siRNA释放，而缓慢降解的纳米球的释放时间变得更长。细胞内siRNA释放较长时间可能有助于延长基因抑制的时间段。(C)2012爱思唯尔有限公司保留所有权利。
The objective of this study is to develop a sustained release system of small interfering RNA (siRNA) inside cells aiming at a prolonged time period of gene suppression. Gelatin aqueous solution containing luciferase siRNA was coacelvated by acetone addition, followed by the glutaraldehyde (GA) crosslinking of gelatin to prepare gelatin nanospheres incorporating siRNA. The nanospheres were degraded with time in phosphate-buffered saline solution containing collagenase to release siRNA incorporated. The nanospheres were degraded more slowly as the GA concentration become higher, and consequently the rate of siRNA become lower. siRNA was released from the nanospheres as a result of nanospheres degradation. The nanospheres were internalized into colon 26 cells luciferase stably expressed, irrespective of the GA concentration. The gene expression was suppressed by the nanospheres incorporating siRNA capable for the longer-term release, and subsequently the time period of gene suppression was prolonged. The siRNA release inside the cell was observed, while the release period became longer for the slow-degraded nanospheres. It is possible that the intracellular siRNA release for a longer time period contributes to the prolonged time period of gene suppression. (C) 2012 Elsevier Ltd. All rights reserved.