Immunohistochemical detection of meconium in the fetal membrane, placenta and umbilical cord
Immunohistochemical detection of meconium in the fetal membrane, placenta and umbilical cord
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DOI：
10.1016/j.placenta.2011.10.007
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发表时间：
2012-01-01
期刊：
影响因子：
3.8
通讯作者：
Kanayama, N.
中科院分区：
文献类型：
--
作者：
Furuta, N.;Yaguchi, C.;Kanayama, N.
目的：为了开发胎粪在胎盘，胎膜和脐带特异性的免疫组化研究design.Study设计：我们以前报道的具体存在锌粪卟啉I（ZnCP-I）在人类胎粪，并证明了可能的诊断使用的母体血浆ZnCP-I水平升高的情况下，羊水栓塞。在这项研究中，我们开发了一种新的特异性单克隆抗体ZnCP-I，并将其应用于免疫组化的胎粪在胎盘，胎膜，脐带。结果：免疫反应ZnCP-I明确和特异性识别胎粪在胎盘，胎膜，脐带。在严重绒毛膜炎的情况下，检测被大量中性粒细胞包围的巨噬细胞中的胎粪特别有用。在超过一半的情况下，胎粪检测在清晰的羊水在delivery，提示以前exposed.Conclusions：ZnCP-I免疫组化检测是一个高度敏感的胎粪的组织学诊断。(C)2011爱思唯尔有限公司保留所有权利。
Objective: To develop the immunohistochemistry specific for meconium in the placenta, fetal membrane and umbilical cord.Study design: We previously reported the specific presence of zinc coproporphyrin I (ZnCP-I) in human meconium and demonstrated the possible diagnostic use of an elevation in maternal plasma ZnCP-I levels in cases of amniotic fluid embolism. In this study, we developed a new specific monoclonal antibody for ZnCP-I and applied it to the immunostaining of meconium in the placenta, fetal membrane, and umbilical cord.Results: lmmunoreactivity of ZnCP-I clearly and specifically identified meconiurn in the placenta, fetal membrane, and umbilical cord. It was especially useful in cases of severe chorioamnionitis to detect meconium in the macrophages surrounded by numerous neutrophils. In more than half of the cases, meconium was detected in clear amniotic fluid at delivery, suggesting previous exposure.Conclusions: lmmunohistochemical detection of ZnCP-I is a highly sensitive histological diagnosis of meconium. (C) 2011 Elsevier Ltd. All rights reserved.