Self-replication of DNA rings
Self-replication of DNA rings
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DOI：
10.1038/nnano.2015.87
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发表时间：
2015-06-01
影响因子：
38.3
通讯作者：
Park, Sung Ha
中科院分区：
文献类型：
--
作者：
Kim, Junghoon;Lee, Junwye;Park, Sung Ha
生物学提供了许多自我复制机器的例子，但人工设计如此复杂的系统仍然是一个艰巨的挑战。特别是，虽然已经设计了简单的人工自我复制系统，包括木块（1,2）、磁性系统（3,4）、模块化机器人（5,6）和合成分子系统（7-9），但与理论细胞自我复制的例子（11-18）相比，这种运动学自我复制器（10）是罕见的。其中一个主要原因是，当您尝试将自我复制合并到物理介质中时，会产生大量的复杂性（19）。在这方面，DNA是构建自我复制系统的主要候选材料，因为它能够通过分子识别进行自组装（20）。在这里，我们展示了DNA t基序，它可以自组装成环状结构（21,22），可以设计成通过支点介导的链位移反应进行自我复制（23,24）。这些环的固有设计允许控制系统的种群动态。我们还分析了自复制的普遍框架内的复制方案（25），并推导了环的自复制性的定量度量。
Biology provides numerous examples of self-replicating machines, but artificially engineering such complex systems remains a formidable challenge. In particular, although simple artificial self-replicating systems including wooden blocks(1,2), magnetic systems(3,4), modular robots(5,6) and synthetic molecular systems(7-9) have been devised, such kinematic self-replicators(10) are rare compared with examples of theoretical cellular selfreplication(11-18). One of the principal reasons for this is the amount of complexity that arises when you try to incorporate self-replication into a physical medium(19). In this regard, DNA is a prime candidate material for constructing self-replicating systems due to its ability to self-assemble through molecular recognition(20). Here, we show that DNA T-motifs, which self-assemble into ring structures(21,22), can be designed to self-replicate through toehold-mediated strand displacement reactions(23,24). The inherent design of these rings allows the population dynamics of the systems to be controlled. We also analyse the replication scheme within a universal framework of self-replication(25) and derive a quantitative metric of the self-replicability of the rings.