MECHANISM LOCALIZATION AND CONTROL OF RESTRICTION CLEAVAGE OF PHAGE-T4 AND LAMBDA CHROMOSOMES INVIVO
MECHANISM LOCALIZATION AND CONTROL OF RESTRICTION CLEAVAGE OF PHAGE-T4 AND LAMBDA CHROMOSOMES INVIVO
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DOI：
10.1038/260406a0
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发表时间：
1976-01-01
期刊：
影响因子：
64.8
通讯作者：
GOLDBERG, EB
中科院分区：
文献类型：
--
作者：
DHARMALINGAM, K;GOLDBERG, EB
阐明了限制性内切酶的初始切割和随后的核酸外切酶V(由rec BC基因指定)在体内裂解片段之间的关系。利用重组BC-细菌[大肠杆菌]，降解从未经修饰的噬菌体T4或噬菌体.lambda裂解的片段。防止了由细胞限制性内切酶引起的染色体损伤。因此，这些大片段显然是体内限制的中间产物，正如体外实验所推测的那样。此外，被认为定位于细胞质或细胞膜中的不同限制性内切酶可以通过不同的方式被感染的噬菌体灭活。
The relationship between the initial cleavage by restriction endonucleases and the subsequent breakdown of the cleaved fragments by exonuclease V (which is specifiied genetically by rec BC) in vivo is clarified. Using rec BC- bacteria [Escherichia coli], degradation of the fragments cleaved from the unmodified phage T4 or phage .lambda. chromosome by the cellular restriction endonucleases was prevented. Therefore these large fragments are apparently intermediates in the restriction in vivo, as postulated after in vitro experiments. Also, different restriction endonucleases which are thought to be localized in the cytoplasm or in the membrane can be inactivated in different ways by the infecting phage.