Synthesis of a cross-linked branched polymer network in the interior of a protein cage.
Synthesis of a cross-linked branched polymer network in the interior of a protein cage.
复制标题
DOI：
10.1021/ja8079862
复制
发表时间：
2009-04-01
影响因子：
15
通讯作者：
Douglas T
中科院分区：
文献类型：
--
作者：
Abedin MJ;Liepold L;Suci P;Young M;Douglas T
仿生化学的一个目标是利用生物系统固有的层次结构来指导功能三维结构的合成。病毒和其他高度对称的蛋白质笼结构提供了定义的支架来启动分层结构组装。在这里，我们证明了交联型支化聚合物可以在蛋白质笼结构的内腔内引发和合成。创建这种聚合物网络允许悬挂式反应部位的空间控制，并极大地提高了笼形结构的稳定性。这种材料是通过使用点击化学将多官能团单体顺序偶联而产生的，从而创建了支化的交联型聚合物网络。用质谱仪分析聚合物的生长情况表明，聚合物是在笼子内表面基因引入的半胱氨酸反应部位引发的。聚合物如预期的那样增长到2.5代，在那里它受到腔的大小限制。聚合物网络通过组成笼子的蛋白质亚单位完全交联，并将笼子的热稳定性扩展到至少120°C。引入的反应中心被证明是活跃的，它们的数密度随着世代的增加而增加。这种合成方法提供了一种新的途径来创建定义的聚合物网络，空间上受生物模板的限制。
A goal of biomimetic chemistry is to use the hierarchical architecture inherent in biological systems to guide the synthesis of functional three dimensional structures. Viruses and other highly symmetrical protein cage architectures provide defined scaffolds to initiate hierarchical structure assembly. Here we demonstrate that a crosslinked branched polymer can be initiated and synthesized within the interior cavity of a protein cage architecture. Creating this polymer network allows for the spatial control of pendant reactive sites and dramatically increases the stability of the cage architecture. This material was generated by the sequential coupling of multifunctional monomers using click chemistry to create a branched crosslinked polymer network. Analysis of polymer growth by mass spectrometry demonstrated that the polymer was initiated at the interior surface of the cage at genetically introduced cysteine reactive sites. The polymer grew as expected to generation 2.5 where it was limited by the size constraints of the cavity. The polymer network was fully crosslinked across protein subunits that make up the cage and extended the thermal stability for the cage to at least 120°C. The introduced reactive centers were shown to be active and their number density increased with increasing generation. This synthetic approach provides a new avenue for creating defined polymer networks, spatially constrained by a biological template.
登录
查看更多内容
影响因子：
15
作者：
Falkner, JC;Turner, ME;Colvin, VL
通讯作者：
Colvin, VL
影响因子：
10.8
作者：
Hooker, Jacob M.;Datta, Ankona;Francis, Matthew B.
通讯作者：
Francis, Matthew B.
影响因子：
15
作者：
Hooker, JM;Kovacs, EW;Francis, MB
通讯作者：
Francis, MB
影响因子：
4.9
作者：
de la Escosura, Andres;Verwegen, Martijn;Cornelissen, Jeroen J. L. M.
通讯作者：
Cornelissen, Jeroen J. L. M.
影响因子：
4.6
作者：
Ensign, D;Young, M;Douglas, T
通讯作者：
Douglas, T