Higher toxicity of nano-scale TiO2 and dose-dependent genotoxicity of nano-scale SiO2 on the cytology and seedling development of broad bean Vicia faba
Higher toxicity of nano-scale TiO2 and dose-dependent genotoxicity of nano-scale SiO2 on the cytology and seedling development of broad bean Vicia faba
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DOI：
10.1007/s42452-019-0960-z
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发表时间：
2019-08
影响因子：
2.6
通讯作者：
Thabet;Galal;El ‐ Samahy;Tuda
中科院分区：
文献类型：
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作者：
Thabet;Galal;El ‐ Samahy;Tuda
自纳米技术进入农业领域以来，其对农作物的安全影响一直是高度优先关注的问题。本研究旨在评价两种不同类型的纳米颗粒（n-）n-SiO2和n-TiO 2对蚕豆（Vicia fabaL.）地上部和地下部生长以及根尖细胞有丝分裂的影响，主要的碳水化合物食物来源之一以及生态毒理学模型植物。用不同浓度（25、50和75 mg/L）的n-SiO2和n-TiO 2浸种24 h。纳米二氧化钛降低活力指数，反映了较短的芽在所有浓度的研究。相反，发芽率和根长不受任何处理。细胞学分析表明，有丝分裂指数（细胞分裂活性指数）与对照组无显著差异。然而，总染色体畸变（%）增加剂量依赖性的n-SiO2和剂量无关的n-TiO 2。此外，纳米材料诱导了不同类型的染色体异常; n-SiO2在50和75 mg/L时诱导桥，而n-TiO 2在50 mg/L时诱导断裂。此外，随着n-SiO2浓度的降低，前期细胞增多，后期细胞减少。我们得出的结论是，在所研究的浓度下，n-TiO 2对蚕豆染色体和早期植物发育的毒性比n-SiO2更大。最后，我们的审查表明，缺乏证据的n-TiO 2在禾本科，一个大的单子叶植物家族，这可能需要进一步关注的发芽增强。
Since nanotechnology entered the field of agriculture, its safety impact on crops has been a high priority interest. Here, we aimed to evaluate the effect of two different types of nanoparticles (n-), n-SiO2and n-TiO2, on the above- and below-ground growth and the root-tip cell mitosis of broad beans (Vicia fabaL.), one of the major carbohydrate food sources as well as an ecotoxicological model plant. Seeds were soaked in n-SiO2and n-TiO2each at different concentrations (25, 50 and 75 mg/L) for 24 h. Nano-TiO2decreased vigor index, reflecting shorter shoots at all concentrations studied. By contrast, germination percentage and root length were not affected by any treatments. Cytological analysis suggested no significant difference in mitotic index (index for cell division activity) from the control. However, total chromosomal aberrations (%) were increased dose-dependently by n-SiO2and dose-independently by n-TiO2. Also, different types of chromosomal abnormalities were induced by the nanomaterials; n-SiO2induced bridges at 50 and 75 mg/L, whereas n-TiO2induced breaks at 50 mg/L. In addition, cells in prophase were more frequently observed and those in anaphase less frequently seen with decreasing n-SiO2concentrations. We concluded that n-TiO2was more toxic than n-SiO2for broad bean chromosomes and early plant development at the concentrations studied. Finally, our review indicates the lack of evidence of germination enhancement by n-TiO2in Poaceae, a large monocotyledon family, which may require further attention.