Simultaneous and bidirectional transport of kinesin‐coated microspheres and dynein‐coated microspheres on polarity‐oriented microtubules
Simultaneous and bidirectional transport of kinesin‐coated microspheres and dynein‐coated microspheres on polarity‐oriented microtubules
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驱动蛋白包被微球和动力蛋白包被微球在极性定向微管上的同时双向运输
DOI：
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发表时间：
2008
影响因子：
3.8
通讯作者：
H. Fujita
中科院分区：
文献类型：
--
作者：
R. Yokokawa;M. Tarhan;T. Kon;H. Fujita
受细胞内转运过程启发的人工纳米转运系统已经使用马达蛋白驱动蛋白和微管研究了十多年。然而，为了在微流体系统中纳米运输的目的，仅实现了单向货物运输。在这里，我们展示了双向纳米运输整合驱动蛋白和动力蛋白马达蛋白。我们的分子系统允许微管在微流体通道中的极性取向，以构建运输轨道。每个马达蛋白都充当纳米致动器，以由定向微管的极性决定的相反方向运输微球：驱动蛋白包被的微球向微管的正端移动，而动力蛋白包被的微球向负端移动。我们使用驱动蛋白和动力蛋白包被的微球在微流体通道中的微管定向和戊二醛固定上证明了单向和双向转运。对微球运动的跟踪和统计分析表明，87-98%的微球在指定方向上运动，对于驱动蛋白包被的微球，平均速度为0.22-0.28 µm/s，对于动力蛋白包被的微球，平均速度为0.34-0.39 µm/s。这种双向纳米转运超越了传统的单向转运，实现了更复杂的体外人工纳米转运。Biotechnol. Biotechnol. Bioeng. 2008;101：1-8.© 2008 Wiley Periodicals，Inc.
Artificial nanotransport systems inspired by intracellular transport processes have been investigated for over a decade using the motor protein kinesin and microtubules. However, only unidirectional cargo transport has been achieved for the purpose of nanotransport in a microfluidic system. Here, we demonstrate bidirectional nanotransport by integrating kinesin and dynein motor proteins. Our molecular system allows microtubule orientation of either polarity in a microfluidic channel to construct a transport track. Each motor protein acts as a nanoactuators that transports microspheres in opposite directions determined by the polarity of the oriented microtubules: kinesin‐coated microspheres move toward the plus end of microtubules, whereas dynein‐coated microspheres move toward the minus end. We demonstrate both unidirectional and bidirectional transport using kinesin‐ and dynein‐coated microspheres on microtubules oriented and glutaraldehyde‐immobilized in a microfluidic channel. Tracking and statistical analysis of microsphere movement demonstrate that 87–98% of microspheres move in the designated direction at a mean velocity of 0.22–0.28 µm/s for kinesin‐coated microspheres and 0.34–0.39 µm/s for dynein‐coated microspheres. This bidirectional nanotransport goes beyond conventional unidirectional transport to achieve more complex artificial nanotransport in vitro. Biotechnol. Biotechnol. Bioeng. 2008;101: 1–8. © 2008 Wiley Periodicals, Inc.
DOI：
10.1073/pnas.92.2.574
发表时间：
1995-01-17
影响因子：
11.1
作者：
MEYHOFER, E;HOWARD, J
通讯作者：
HOWARD, J