EFFECTS OF STARVATION AND NEUROACTIVE DRUGS ON FEEDING IN CAENORHABDITIS-ELEGANS
EFFECTS OF STARVATION AND NEUROACTIVE DRUGS ON FEEDING IN CAENORHABDITIS-ELEGANS
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DOI：
10.1002/jez.1402530305
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发表时间：
1990-03-01
影响因子：
--
通讯作者：
HORVITZ, HR
中科院分区：
文献类型：
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作者：
AVERY, L;HORVITZ, HR
秀丽隐杆线虫通过咽部的抽吸来集中食物细菌。泵送速率受细菌存在的影响。使用一种新的检测方法，允许测量泵的速度在人口的蠕虫悬浮在液体中，通过测量他们的微观铁颗粒的吸收，我们已经证实和量化这种效果。此外，我们证明了饥饿刺激泵。蠕虫已经剥夺了细菌超过4小时，在没有细菌的情况下泵入，而喂养良好的蠕虫则没有。此外，饥饿的蠕虫比喂养的蠕虫对较低数量的细菌作出反应。该测定也可用于测量药物对泵送的影响。在筛选的大约30种化学物质中，有5种具有明显的效果。神经递质5-羟色胺和5-羟色胺摄取抑制剂丙咪嗪刺激泵，而5-羟色胺拮抗剂gramine抑制。丙咪嗪刺激在cat-1和cat-4突变体中大大降低，它们具有低水平的5-羟色胺。蝇蕈醇是一种神经递质GABA的激动剂，伊维菌素的作用部位也可能是GABA受体，两者都抑制泵。定性观察表明，乙酰胆碱在泵的调节作用。
Caenorhabditis elegans concentrates its food, bacteria, by pharyngeal pumping. The rate of pumping is affected by the presence of bacteria. Using a new assay that allows measurement of pumping rate in a population of worms suspended in liquid by measuring their uptake of microscopic iron particles, we have confirmed and quantitated this effect. Furthermore, we demonstrated that starvation stimulates pumping. Worms that had been deprived of bacteria for more than 4 hours pumped in the absence of bacteria under conditions in which well-fed worms did not. Furthermore, starved worms responded to lower amounts of bacteria than did fed worms. The assay was also useful for measuring effects of drugs on pumping. Of about 30 chemicals screened, 5 had clear effects. The neurotransmitter serotonin and the serotonin uptake inhibitor imipramine stimulated pumping, while the serotonin antagonist gramine inhibits. Imipramine stimulation is greatly decreased in cat-1 and cat-4 mutants, which have low levels of serotonin. Muscimol, an agonist for the neurotransmitter GABA, and ivermectin, whose site of action may also be the GABA receptor, both inhibit pumping. Qualitative observations suggested a role for acetylcholine in the regulation of pumping.