Molecular and physiological characterization of Arabidopsis-Colletotrichum gloeosporioides pathosystem
Molecular and physiological characterization of Arabidopsis-Colletotrichum gloeosporioides pathosystem
复制标题
胶孢炭疽菌致病系统的分子生物学特性
DOI：
10.1111/ppa.13364
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发表时间：
2021-03-09
期刊：
影响因子：
2.7
通讯作者：
Luo, Lijuan
中科院分区：
文献类型：
--
作者：
Gao, Mengze;Wan, Miting;Luo, Lijuan
炭疽病是一种毁灭性的疾病，影响范围广泛的作物，特别是在热带和亚热带地区。炭疽菌是引起炭疽病的主要病原体。本研究从热带主要饲料作物柱花草的病害样品中采集病原菌并进行鉴定。检测了病原体天然感染拟南芥的能力。ITS、ACT、CHS和GAPDH基因序列分析表明该病原菌为广义胶孢炭疽菌（Colletotrichum gloeosporioides s.l.），并且这进一步得到代表性分离物的形态学表征的支持。比较了强毒株（DZ-19和HK-04）和弱毒株（CJ-04）的症状和细胞侵染过程。与CJ-04相比，DZ-19和HK-04对Col-0和Ws-2生态类型的幼苗和成年植株的病害症状都更严重。在侵染过程中，较强的菌株分生孢子萌发、附着胞形成和菌丝生长发育的时间更快、更早。防御反应和防御标记基因的表达谱的遗传分析表明，参与MAP激酶，Ca 2+依赖性蛋白激酶，水杨酸，乙烯和茉莉酸途径的抗炭疽病。这些结果表明，炭疽病-炭疽病病原系统应该提供一个有价值的工具，探索对该病原菌的抗性机制。
Anthracnose is a destructive disease that affects a wide range of crop plants especially in tropical and subtropical regions. Colletotrichum spp. are the major pathogens causing anthracnose. In this study, we collected and identified the pathogen from diseased samples of Stylosanthes, a major tropical forage crop. The ability of the pathogen to naturally infect Arabidopsis thaliana was examined. Sequence analysis of ITS, ACT, CHS, and GAPDH genes showed the pathogen to be Colletotrichum gloeosporioides sensu lato (s.l.), and this was supported further by morphological characterization of representative isolates. The disease symptoms and cellular infection process of aggressive isolates (DZ-19 and HK-04) and a weak isolate (CJ-04) were compared. DZ-19 and HK-04 caused more severe disease symptoms on both young seedlings and adult plants of Col-0 and Ws-2 ecotypes compared to CJ-04. Furthermore, the more aggressive isolates showed faster and earlier germination of conidia, formation of appressoria, and growth and development of hyphae during the infection. Genetic analysis of the defence response and expression profiling of defence marker genes demonstrated the involvement of MAP kinase, Ca2+-dependent protein kinase, salicylic acid, ethylene, and jasmonic acid pathways in the resistance against anthracnose. These results suggest that the Arabidopsis-Colletotrichum gloeosporioides pathosystem should provide a valuable tool for exploring the resistance mechanisms against this pathogen.